Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil

Hydrocarbon-degrading bacteria, which can be found living with eukaryotic phytoplankton, play a pivotal role in the fate of oil spillage to the marine environment. Considering the susceptibility of calcium carbonate-bearing phytoplankton under future ocean acidification conditions and their oil-degr...

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Main Authors: Fahmi, Afiq Mohd, Summers, Stephen, Jones, Martin, Bowler, Bernard, Hennige, Sebastian, Gutierrez, Tony
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/169385
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spelling sg-ntu-dr.10356-1693852023-07-17T15:31:58Z Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil Fahmi, Afiq Mohd Summers, Stephen Jones, Martin Bowler, Bernard Hennige, Sebastian Gutierrez, Tony School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering Engineering::Environmental engineering Bacteria Carbon Dioxide Hydrocarbon-degrading bacteria, which can be found living with eukaryotic phytoplankton, play a pivotal role in the fate of oil spillage to the marine environment. Considering the susceptibility of calcium carbonate-bearing phytoplankton under future ocean acidification conditions and their oil-degrading communities to oil exposure under such conditions, we investigated the response of non-axenic E. huxleyi to crude oil under ambient versus elevated CO2 concentrations. Under elevated CO2 conditions, exposure to crude oil resulted in the immediate decline of E. huxleyi, with concomitant shifts in the relative abundance of Alphaproteobacteria and Gammaproteobacteria. Survival of E. huxleyi under ambient conditions following oil enrichment was likely facilitated by enrichment of oil-degraders Methylobacterium and Sphingomonas, while the increase in relative abundance of Marinobacter and unclassified Gammaproteobacteria may have increased competitive pressure with E. huxleyi for micronutrient acquisition. Biodegradation of the oil was not affected by elevated CO2 despite a shift in relative abundance of known and putative hydrocarbon degraders. While ocean acidification does not appear to affect microbial degradation of crude oil, elevated mortality responses of E. huxleyi and shifts in the bacterial community illustrates the complexity of microalgal-bacterial interactions and highlights the need to factor these into future ecosystem recovery projections. Published version This manuscript contains work conducted during a PhD study sponsored by a studentship to AMF from the University of Malaysia, Terengganu (UMT) and whose support is gratefully acknowledged. Support was also provided through two Natural Environment Research Council grants (NERC, NE/K009028/1 and NE/K009028/2) to SH. 2023-07-17T06:40:30Z 2023-07-17T06:40:30Z 2023 Journal Article Fahmi, A. M., Summers, S., Jones, M., Bowler, B., Hennige, S. & Gutierrez, T. (2023). Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil. Scientific Reports, 13(1), 5013-. https://dx.doi.org/10.1038/s41598-023-31784-5 2045-2322 https://hdl.handle.net/10356/169385 10.1038/s41598-023-31784-5 36973465 2-s2.0-85150958833 1 13 5013 en Scientific Reports © 2023 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Bacteria
Carbon Dioxide
spellingShingle Engineering::Environmental engineering
Bacteria
Carbon Dioxide
Fahmi, Afiq Mohd
Summers, Stephen
Jones, Martin
Bowler, Bernard
Hennige, Sebastian
Gutierrez, Tony
Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
description Hydrocarbon-degrading bacteria, which can be found living with eukaryotic phytoplankton, play a pivotal role in the fate of oil spillage to the marine environment. Considering the susceptibility of calcium carbonate-bearing phytoplankton under future ocean acidification conditions and their oil-degrading communities to oil exposure under such conditions, we investigated the response of non-axenic E. huxleyi to crude oil under ambient versus elevated CO2 concentrations. Under elevated CO2 conditions, exposure to crude oil resulted in the immediate decline of E. huxleyi, with concomitant shifts in the relative abundance of Alphaproteobacteria and Gammaproteobacteria. Survival of E. huxleyi under ambient conditions following oil enrichment was likely facilitated by enrichment of oil-degraders Methylobacterium and Sphingomonas, while the increase in relative abundance of Marinobacter and unclassified Gammaproteobacteria may have increased competitive pressure with E. huxleyi for micronutrient acquisition. Biodegradation of the oil was not affected by elevated CO2 despite a shift in relative abundance of known and putative hydrocarbon degraders. While ocean acidification does not appear to affect microbial degradation of crude oil, elevated mortality responses of E. huxleyi and shifts in the bacterial community illustrates the complexity of microalgal-bacterial interactions and highlights the need to factor these into future ecosystem recovery projections.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Fahmi, Afiq Mohd
Summers, Stephen
Jones, Martin
Bowler, Bernard
Hennige, Sebastian
Gutierrez, Tony
format Article
author Fahmi, Afiq Mohd
Summers, Stephen
Jones, Martin
Bowler, Bernard
Hennige, Sebastian
Gutierrez, Tony
author_sort Fahmi, Afiq Mohd
title Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
title_short Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
title_full Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
title_fullStr Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
title_full_unstemmed Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
title_sort effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
publishDate 2023
url https://hdl.handle.net/10356/169385
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