Ribosomal proteins regulate 2-cell-stage transcriptome in mouse embryonic stem cells

Ribosomal proteins regulate 2-cell-stage transcriptome in mouse embryonic stem cells

A rare sub-population of mouse embryonic stem cells (mESCs), the 2-cell-like cell, is defined by the expression of MERVL and 2-cell-stage-specific transcript (2C transcript). Here, we report that the ribosomal proteins (RPs) RPL14, RPL18, and RPL23 maintain the identity of mESCs and regulate the exp...

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Bibliographic Details
Main Authors: Yi, Yao, Zeng, Yingying, Sam, Tsz Wing, Hamashima, Kiyofumi, Tan, Rachel Jun Rou, Warrier, Tushar, Phua, Jun Xiang, Taneja, Reshma, Liou, Yih-Cherng, Li, Hu, Xu, Jian, Loh, Yuin-Han
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
Subjects:
Science::Biological sciences
Online Access:https://hdl.handle.net/10356/169689
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Institution: Nanyang Technological University
Language: English
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Summary:A rare sub-population of mouse embryonic stem cells (mESCs), the 2-cell-like cell, is defined by the expression of MERVL and 2-cell-stage-specific transcript (2C transcript). Here, we report that the ribosomal proteins (RPs) RPL14, RPL18, and RPL23 maintain the identity of mESCs and regulate the expression of 2C transcripts. Disregulation of the RPs induces DUX-dependent expression of 2C transcripts and alters the chromatin landscape. Mechanically, knockdown (KD) of RPs triggers the binding of RPL11 to MDM2, an interaction known to prevent P53 protein degradation. Increased P53 protein upon RP KD further activates its downstream pathways, including DUX. Our study delineates the critical roles of RPs in 2C transcript activation, ascribing a novel function to these essential proteins.

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