Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells

In a previous study in our lab, we found that the expression of exogenous EZH2 in the cytosol contributes to colony formation in H16N2 cell lines, and increased tumour growth in both immune-deficient and immune-competent mouse models. Therefore, we hypothesised that cancer stem cells from TNBC tumou...

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Main Author: Khalilatul Hanisah Binte Mohd Kahliab
Other Authors: Su I-Hsin
Format: Thesis-Master by Research
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/169785
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spelling sg-ntu-dr.10356-1697852023-09-04T07:32:08Z Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells Khalilatul Hanisah Binte Mohd Kahliab Su I-Hsin School of Biological Sciences IHSu@ntu.edu.sg Science::Biological sciences::Molecular biology In a previous study in our lab, we found that the expression of exogenous EZH2 in the cytosol contributes to colony formation in H16N2 cell lines, and increased tumour growth in both immune-deficient and immune-competent mouse models. Therefore, we hypothesised that cancer stem cells from TNBC tumours should have increased cytosolic EZH2 due to the aggressiveness of this cancer compared to the different breast cancer subtypes. To investigate if this phenomenon is true, we employed a BLG-Cre; Brca1F22–24; p53 KO mouse model to investigate cytosolic EZH2 expression in TNBC tumours. Indeed, upon enriching YFP+ BRCA1-deficient cancer cells from our TNBC tumours, we observed the YFP+ CD44+ BRCA1-deficient cancer cells exhibited increased cytosolic EZH2 compared to YFP+ CD44– BRCA1-deficient cancer cells. We also monitored the immune cell changes in the blood of LGB-Cre+p53+/–Brca1fl/fl YFP/YFP mice to predict cancer progression and tumour formation since spontaneous TNBC tumours require extended incubation times to develop. Upon monitoring the immune cell changes in the blood at different time points, we observed immune cells change over time from a pro-inflammatory to an anti-inflammatory phenotype to promote tumorigenesis. Master of Science 2023-08-04T00:58:50Z 2023-08-04T00:58:50Z 2023 Thesis-Master by Research Khalilatul Hanisah Binte Mohd Kahliab (2023). Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells. Master's thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/169785 https://hdl.handle.net/10356/169785 10.32657/10356/169785 en 04MNP001248C220MAC01 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences::Molecular biology
spellingShingle Science::Biological sciences::Molecular biology
Khalilatul Hanisah Binte Mohd Kahliab
Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
description In a previous study in our lab, we found that the expression of exogenous EZH2 in the cytosol contributes to colony formation in H16N2 cell lines, and increased tumour growth in both immune-deficient and immune-competent mouse models. Therefore, we hypothesised that cancer stem cells from TNBC tumours should have increased cytosolic EZH2 due to the aggressiveness of this cancer compared to the different breast cancer subtypes. To investigate if this phenomenon is true, we employed a BLG-Cre; Brca1F22–24; p53 KO mouse model to investigate cytosolic EZH2 expression in TNBC tumours. Indeed, upon enriching YFP+ BRCA1-deficient cancer cells from our TNBC tumours, we observed the YFP+ CD44+ BRCA1-deficient cancer cells exhibited increased cytosolic EZH2 compared to YFP+ CD44– BRCA1-deficient cancer cells. We also monitored the immune cell changes in the blood of LGB-Cre+p53+/–Brca1fl/fl YFP/YFP mice to predict cancer progression and tumour formation since spontaneous TNBC tumours require extended incubation times to develop. Upon monitoring the immune cell changes in the blood at different time points, we observed immune cells change over time from a pro-inflammatory to an anti-inflammatory phenotype to promote tumorigenesis.
author2 Su I-Hsin
author_facet Su I-Hsin
Khalilatul Hanisah Binte Mohd Kahliab
format Thesis-Master by Research
author Khalilatul Hanisah Binte Mohd Kahliab
author_sort Khalilatul Hanisah Binte Mohd Kahliab
title Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
title_short Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
title_full Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
title_fullStr Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
title_full_unstemmed Exploring the immune profile in a TNBC mouse model while elucidating the cytosolic EZH2 expression in cancer stem cells
title_sort exploring the immune profile in a tnbc mouse model while elucidating the cytosolic ezh2 expression in cancer stem cells
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/169785
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