Optimising expression and purification of Dengue soluble E protein
This study explores the significance of expression and purification of soluble dengue Envelope (E) protein which has strong applications in viral biology, vaccine development, and therapeutic strategies. Dengue, a type of flavivirus, is a widespread mosquito-borne infection lacking effective antivir...
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| Format: | Final Year Project |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/169794 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | This study explores the significance of expression and purification of soluble dengue Envelope (E) protein which has strong applications in viral biology, vaccine development, and therapeutic strategies. Dengue, a type of flavivirus, is a widespread mosquito-borne infection lacking effective antiviral treatments. The E protein is crucial for viral fusion and host cell entry. The soluble E (sE) protein, obtained by truncation of Transmembrane Domain (TMD), serves as an antigen for serological assays, aiding diagnosis and seroprevalence assessment. It offers insights into viral-host immune interactions. The full-length E protein is essential for vaccine design. The study aims to express and purify the soluble E protein, modified with Tobacco Etch Virus (TEV) and Twin-Strep-tag®, for applications such as structural study and high throughput screening. Successful modification and vector expression in pcDNA3.1 enhance solubility, enabling drug development and testing. This project sets a baseline for future functional and mechanistic understanding. Overall, it advances understanding of Dengue virus biology and potential therapeutic advances. |
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