Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion

Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion

With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galact...

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Main Author: Teng, Rou Yi
Other Authors: Xiao Tianshu
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
Subjects:
Science::Biological sciences
Online Access:https://hdl.handle.net/10356/169795
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1697952023-09-12T08:22:38Z Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion Teng, Rou Yi Xiao Tianshu School of Biological Sciences tianshu.xiao@ntu.edu.sg Science::Biological sciences With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galactosidase chemiluminescence. Although initial results were not the most favourable, adjustments in subsequent rounds have improved results. Experimental parameters will continue to be adjusted to further improve results, with hopes to move onto inhibitor studies. Bachelor of Science in Biomedical Sciences 2023-08-04T02:18:58Z 2023-08-04T02:18:58Z 2023 Final Year Project (FYP) Teng, R. Y. (2023). Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/169795 https://hdl.handle.net/10356/169795 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Teng, Rou Yi
Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
description With the Dengue virus being such a prominent problem in the world today, this project focuses on the dengue virus and its E protein, which is the key for its entry into cells. This project aims to optimise a fusion assay using two methods: the first is by GFP/RFP fluorescence, the second by -galactosidase chemiluminescence. Although initial results were not the most favourable, adjustments in subsequent rounds have improved results. Experimental parameters will continue to be adjusted to further improve results, with hopes to move onto inhibitor studies.
author2 Xiao Tianshu
author_facet Xiao Tianshu
Teng, Rou Yi
format Final Year Project
author Teng, Rou Yi
author_sort Teng, Rou Yi
title Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
title_short Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
title_full Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
title_fullStr Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
title_full_unstemmed Developing a cell-based assay to quantify and visualise dengue E protein-mediated membrane fusion
title_sort developing a cell-based assay to quantify and visualise dengue e protein-mediated membrane fusion
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/169795
_version_ 1779156597804630016

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