Development of a novel design of microfluidic impedance cytometry for improved sensitivity and cell identification

• Main Authors: Warren, Michael A., Shakouri, Amir, Pacheco-Peña, Víctor, Hallam, Toby
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Physics; Flow Cytometry; Optofluidics
• Online Access: https://hdl.handle.net/10356/169882
• Institution: Nanyang Technological University

A long-standing issue for microfluidic impedance cytometry devices is the accuracy in determining the size of cells during counting and measurements. In this paper, we introduce a novel design that produces a homogeneous electric field in the sensing region and demonstrates higher accuracy than traditional designs in cell counting and sizing, reducing the reliance on cell focusing and signal postprocessing. The concept is validated, and the increased accuracy of the device over traditional designs is demonstrated through the use of finite element simulations to generate suitable data sets for particle trajectories and model expected signal variations.