Label-free single cell impedance analysis of IPSC-derived spinal cord progenitor cells for rapid profiling of safety and efficacy phenotypes

• Main Authors: Tan, Jerome Zu Yao, He, Linwei, Ng, Shi-Yan, Li, Holden King Ho, Hou, Han Wei, Chew, Sing Yian, Han, Jongyoon
• Other Authors: Interdisciplinary Graduate School (IGS)
• Format: Conference or Workshop Item
• Language: English
• Published: 2023
• Subjects: Engineering; Cell Therapy; Microfluidics; Biomanufacturing; Impedance
• Online Access: https://hdl.handle.net/10356/170115; https://ap2023.termis.org/
• Institution: Nanyang Technological University

The use of induced pluripotent stem cells (iPSCs) to derive progenitor cells for transplantations has garnered huge interest because of its strong potential to repair damaged tissue, especially after spinal cord injuries. However, the differentiation of iPSCs into neurons and neural cell types have been highly variable and requires reliable assessment of its differentiation efficiency, to validate both its safety and quality. Phenotyping is often performed via label-based methods including immunofluorescent staining or flow cytometry analysis. These approaches are often expensive, laborious, time-consuming, destructive, and severely limits their use in large scale cell therapy manufacturing settings. On the other hand, cellular biophysical properties have demonstrated a strong correlation to cell state, quality and functionality and can be measured with ingenious microfluidic label-free technologies in a rapid and non-destructive manner.