Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling

Myrosinase (Myr) is a type of critical β-thioglucosidase enzyme activator essential for sustaining many functional foods to perform their health-promoting functions. An accurate and reliable Myr test is meaningful for food quality and dietary nutrition assessments, whereas the currently reported met...

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Main Authors: Qiao, Ling, Lang, Wenchao, Sun, Caixia, Huang, Yining, Wu, Ping, Cai, Chenxin, Xing, Bengang
Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/170566
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1705662023-11-02T01:44:05Z Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling Qiao, Ling Lang, Wenchao Sun, Caixia Huang, Yining Wu, Ping Cai, Chenxin Xing, Bengang School of Chemistry, Chemical Engineering and Biotechnology Science::Chemistry Colorimetric Biosensors Myrosinase Myrosinase (Myr) is a type of critical β-thioglucosidase enzyme activator essential for sustaining many functional foods to perform their health-promoting functions. An accurate and reliable Myr test is meaningful for food quality and dietary nutrition assessments, whereas the currently reported methods do not guarantee specificity and have high reliance on instrumentation, which are not suitable for rapid and onsite Myr screening especially in complex systems from various sources. Herein, we present a unique NIR-II absorption-based photothermal-responsive colorimetric biosensor for anti-interference onsite Myr determination and realization of rapid visualized outputs with the aid of smartphone calculation. Typically, assisted by glucose oxidase (GOx), Myr specifically converts the sinigrin substrate into hydrogen peroxide (H2O2) that can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by AuNPs to form a charge transfer complex (CTC) with NIR-II absorption and photothermal characters. Delightfully, such a proposed method is able to determine Myr within a wide range of 0 to 172.5 mU/mL with a detection limit down to 2.96 mU/mL. Moreover, simple, rapid, and real-time visual Myr identification in actual food-sourced samples could also be readily achieved by smartphone readout processing, with the promising advantages of anti-interference, high accuracy, and low cost as well as labor-saving and intelligence engagement, thus providing great feasibility for precise measurement in complex and dynamic dietary sample analysis. Overall, our proposed method presents a novel technology for onsite dietary Myr enzyme profiling, which is promising to be applied in the food industry for nutritional composition profiles, freshness evaluation, and quality assessment. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) This work is supported by MOE Tier 1 RG4/22, RG6/20, A*Star SERC A1983c0028, A20E5c0090, awarded at Nanyang Technological University (NTU), and the National Natural Science Foundation of China (NSFC) (No. 51929201). L.Q. gratefully acknowledges financial support from the China Scholarship Council (NO.202006860052) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX21-1327). 2023-09-19T06:20:00Z 2023-09-19T06:20:00Z 2023 Journal Article Qiao, L., Lang, W., Sun, C., Huang, Y., Wu, P., Cai, C. & Xing, B. (2023). Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling. Analytical Chemistry, 95(7), 3856-3863. https://dx.doi.org/10.1021/acs.analchem.2c05474 0003-2700 https://hdl.handle.net/10356/170566 10.1021/acs.analchem.2c05474 36756955 2-s2.0-85148045808 7 95 3856 3863 en RG4/22 RG6/20 A1983c0028 A20E5c0090 Analytical Chemistry © 2023 American Chemical Society. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
Colorimetric Biosensors
Myrosinase
spellingShingle Science::Chemistry
Colorimetric Biosensors
Myrosinase
Qiao, Ling
Lang, Wenchao
Sun, Caixia
Huang, Yining
Wu, Ping
Cai, Chenxin
Xing, Bengang
Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
description Myrosinase (Myr) is a type of critical β-thioglucosidase enzyme activator essential for sustaining many functional foods to perform their health-promoting functions. An accurate and reliable Myr test is meaningful for food quality and dietary nutrition assessments, whereas the currently reported methods do not guarantee specificity and have high reliance on instrumentation, which are not suitable for rapid and onsite Myr screening especially in complex systems from various sources. Herein, we present a unique NIR-II absorption-based photothermal-responsive colorimetric biosensor for anti-interference onsite Myr determination and realization of rapid visualized outputs with the aid of smartphone calculation. Typically, assisted by glucose oxidase (GOx), Myr specifically converts the sinigrin substrate into hydrogen peroxide (H2O2) that can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by AuNPs to form a charge transfer complex (CTC) with NIR-II absorption and photothermal characters. Delightfully, such a proposed method is able to determine Myr within a wide range of 0 to 172.5 mU/mL with a detection limit down to 2.96 mU/mL. Moreover, simple, rapid, and real-time visual Myr identification in actual food-sourced samples could also be readily achieved by smartphone readout processing, with the promising advantages of anti-interference, high accuracy, and low cost as well as labor-saving and intelligence engagement, thus providing great feasibility for precise measurement in complex and dynamic dietary sample analysis. Overall, our proposed method presents a novel technology for onsite dietary Myr enzyme profiling, which is promising to be applied in the food industry for nutritional composition profiles, freshness evaluation, and quality assessment.
author2 School of Chemistry, Chemical Engineering and Biotechnology
author_facet School of Chemistry, Chemical Engineering and Biotechnology
Qiao, Ling
Lang, Wenchao
Sun, Caixia
Huang, Yining
Wu, Ping
Cai, Chenxin
Xing, Bengang
format Article
author Qiao, Ling
Lang, Wenchao
Sun, Caixia
Huang, Yining
Wu, Ping
Cai, Chenxin
Xing, Bengang
author_sort Qiao, Ling
title Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
title_short Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
title_full Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
title_fullStr Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
title_full_unstemmed Near infrared-II photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
title_sort near infrared-ii photothermal and colorimetric synergistic sensing for intelligent onsite dietary myrosinase profiling
publishDate 2023
url https://hdl.handle.net/10356/170566
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