Dual strategy for ¹³C-Metabolic flux analysis of central carbon and energy metabolism in mammalian cells based on LC-isoMRM-MS

• Main Authors: Zheng, Jie, Yang, Junjie, Liang, Xu, Fang, Mingliang, Wang, Yulan
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Medicine; Science::Biological sciences; Central Carbon and Energy Metabolism; Carboxyl and Phosphate Metabolites
• Online Access: https://hdl.handle.net/10356/171182
• Institution: Nanyang Technological University

Central carbon and energy metabolism are the most concerned metabolic pathways in 13C-Metabolic flux analysis (13C-MFA). However, some α-keto acids, ribonucleoside triphosphate (NTPs) and deoxyribonucleoside triphosphate (dNTPs) involved in central carbon and energy metabolism pathways were unstable...