Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli
NG-Test CARBA 5 (NG-Biotech) is a rapid in vitro multiplex immunoassay for the phenotypic detection and differentiation of the "big five" carbapenemase families (KPC, OXA-48-like, VIM, IMP, and NDM). Version 2 of this assay was evaluated alongside the Xpert Carba-R assay (Cepheid, Inc.), t...
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sg-ntu-dr.10356-1715892023-11-05T15:39:50Z Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli Khoo, Bo Yan Hon, Pei Yun Leong, Janice Sridatta, Prakki Sai Rama Thevasagayam, Natascha May Loy, Dennis Song Qi Chua, Jasmine J. Y. Ang, Brenda Sze Peng Chow, Angela Marimuthu, Kalisvar De, Partha Pratim Ng, Oon Tek Vasoo, Shawn Lee Kong Chian School of Medicine (LKCMedicine) National Centre for Infectious Diseases Tan Tock Seng Hospital Yong Loo Lin School of Medicine, NUS Saw Swee Hock School of Public Health, NUS Science::Medicine NG-Test CARBA 5 Xpert Carba-R NG-Test CARBA 5 (NG-Biotech) is a rapid in vitro multiplex immunoassay for the phenotypic detection and differentiation of the "big five" carbapenemase families (KPC, OXA-48-like, VIM, IMP, and NDM). Version 2 of this assay was evaluated alongside the Xpert Carba-R assay (Cepheid, Inc.), the modified carbapenem inactivation method (mCIM), and the CIMTris assay, with a collection of carbapenem-resistant non-fermenting Gram-negative bacilli comprising 138 Pseudomonas aeruginosa and 97 Acinetobacter baumannii isolates. Whole-genome sequencing (WGS) was used as the reference standard. For P. aeruginosa, NG-Test CARBA 5 produced an overall percentage agreement (OPA) with WGS of 97.1%, compared with 92.8% forXpert Carba-R and 90.6% for mCIM. For A. baumannii, as OXA-type carbapenemases (non-OXA-48) are not included, both the NG-Test CARBA 5 and Xpert Carba-R only had an OPA of 6.2%, while the CIMTris performed well with an OPA of 99.0%. The majority of A. baumannii isolates (95.9%) tested falsely positive for IMP on NG-Test CARBA 5; no IMP genes were found on WGS. No clear cause was found for this phenomenon; a cross-reacting protein antigen unique to A. baumannii is a possible culprit. NG-Test CARBA 5 performed well for carbapenemase detection in P. aeruginosa. However, results from A. baumannii isolates should be interpreted with caution. Ministry of Health (MOH) National Medical Research Council (NMRC) Published version This work was supported by the Ministry of Health, Singapore, under the FY16 Health Service Development Programme (HSDP) Project 19N01: "Reducing the spread of carbapenemase producing Gram-negative bacteria via rapid and direct detection from surveillance and clinical samples" (S.V.), as well as the National Medical Research Council (NMRC) Centre Grant: “Collaborative Solutions Targeting Antimicrobial Resistance Threats in Health Systems (CoSTAR-HS)" (CG21APR2005), NMRC Clinician Scientist Award (MOH-000276), NMRC Open Fund—Large Collaborative Grant: AntiMicrobial resistance Research & Intervention Alliance Singapore (AMRITAS; MOH-001326-01) (O.T.N.), and NMRC Clinician Scientist Individual Research Grant (CIRG18Nov-0034) (K.M.). 2023-10-31T08:01:01Z 2023-10-31T08:01:01Z 2023 Journal Article Khoo, B. Y., Hon, P. Y., Leong, J., Sridatta, P. S. R., Thevasagayam, N. M., Loy, D. S. Q., Chua, J. J. Y., Ang, B. S. P., Chow, A., Marimuthu, K., De, P. P., Ng, O. T. & Vasoo, S. (2023). Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli. Journal of Clinical Microbiology, 61(9), e0031623-. https://dx.doi.org/10.1128/jcm.00316-23 0095-1137 https://hdl.handle.net/10356/171589 10.1128/jcm.00316-23 37671882 2-s2.0-85171901878 9 61 e0031623 en 19N01 CG21APR2005 MOH-000276 MOH-001326-01 Journal of Clinical Microbiology © 2023 American Society for Microbiology. All Rights Reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1128/jcm.00316-23 application/pdf |
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Science::Medicine NG-Test CARBA 5 Xpert Carba-R Khoo, Bo Yan Hon, Pei Yun Leong, Janice Sridatta, Prakki Sai Rama Thevasagayam, Natascha May Loy, Dennis Song Qi Chua, Jasmine J. Y. Ang, Brenda Sze Peng Chow, Angela Marimuthu, Kalisvar De, Partha Pratim Ng, Oon Tek Vasoo, Shawn Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
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NG-Test CARBA 5 (NG-Biotech) is a rapid in vitro multiplex immunoassay for the phenotypic detection and differentiation of the "big five" carbapenemase families (KPC, OXA-48-like, VIM, IMP, and NDM). Version 2 of this assay was evaluated alongside the Xpert Carba-R assay (Cepheid, Inc.), the modified carbapenem inactivation method (mCIM), and the CIMTris assay, with a collection of carbapenem-resistant non-fermenting Gram-negative bacilli comprising 138 Pseudomonas aeruginosa and 97 Acinetobacter baumannii isolates. Whole-genome sequencing (WGS) was used as the reference standard. For P. aeruginosa, NG-Test CARBA 5 produced an overall percentage agreement (OPA) with WGS of 97.1%, compared with 92.8% forXpert Carba-R and 90.6% for mCIM. For A. baumannii, as OXA-type carbapenemases (non-OXA-48) are not included, both the NG-Test CARBA 5 and Xpert Carba-R only had an OPA of 6.2%, while the CIMTris performed well with an OPA of 99.0%. The majority of A. baumannii isolates (95.9%) tested falsely positive for IMP on NG-Test CARBA 5; no IMP genes were found on WGS. No clear cause was found for this phenomenon; a cross-reacting protein antigen unique to A. baumannii is a possible culprit. NG-Test CARBA 5 performed well for carbapenemase detection in P. aeruginosa. However, results from A. baumannii isolates should be interpreted with caution. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
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Lee Kong Chian School of Medicine (LKCMedicine) Khoo, Bo Yan Hon, Pei Yun Leong, Janice Sridatta, Prakki Sai Rama Thevasagayam, Natascha May Loy, Dennis Song Qi Chua, Jasmine J. Y. Ang, Brenda Sze Peng Chow, Angela Marimuthu, Kalisvar De, Partha Pratim Ng, Oon Tek Vasoo, Shawn |
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Article |
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Khoo, Bo Yan Hon, Pei Yun Leong, Janice Sridatta, Prakki Sai Rama Thevasagayam, Natascha May Loy, Dennis Song Qi Chua, Jasmine J. Y. Ang, Brenda Sze Peng Chow, Angela Marimuthu, Kalisvar De, Partha Pratim Ng, Oon Tek Vasoo, Shawn |
author_sort |
Khoo, Bo Yan |
title |
Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
title_short |
Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
title_full |
Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
title_fullStr |
Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
title_full_unstemmed |
Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli |
title_sort |
evaluation of ng-test carba 5 version 2, cepheid xpert carba-r, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting gram-negative bacilli |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/171589 |
_version_ |
1783955606549299200 |