Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore
Pseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as blaIMP and blaVIM but has been rarely associated with blaNDM-1. A total of 199 P. aeruginosa ST308 clinical and environmental isolates obtained between April 2019 and November 2020 from a tertiary-care hospital...
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sg-ntu-dr.10356-1716372023-11-05T15:39:21Z Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore Prakki, Sai Rama Sridatta Hon, Pei Yun Lim, Ze Qin Thevasagayam, Natascha May Loy, Song Qi Dennis De, Partha Pratim Marimuthu, Kalisvar Vasoo, Shawn Ng, Oon Tek Lee Kong Chian School of Medicine (LKCMedicine) National Centre for Infectious Diseases Tan Tock Seng Hospital Science::Medicine Carbapenemase Pseudomonas Aeruginosa Pseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as blaIMP and blaVIM but has been rarely associated with blaNDM-1. A total of 199 P. aeruginosa ST308 clinical and environmental isolates obtained between April 2019 and November 2020 from a tertiary-care hospital in Singapore were characterized using whole-genome sequencing. In addition, 71 blaNDM-1-positive ST308 whole-genome sequences from two other local tertiary-care hospitals in Singapore and 83 global blaNDM-1-negative ST308 whole-genome sequences in public databases were included to assess phylogenetic relationships and perform genome analyses. Phylogenetic analysis and divergent time estimation revealed that blaNDM-1-positive P. aeruginosa ST308 was introduced into Singapore in 2005 (95 % highest posterior density: 2001 to 2008). Core genome, resistome, and analyses of all local blaNDM-1-positive ST308 isolates showed chromosomal integration of multiple antibiotic resistance genes (ARGs) [aac(3)-Id, aac(6')-Il, aadA6, aadA11, dfrB5, msr(E), floR, sul2, and qnrVC1], which was absent in global blaNDM-1-negative ST308 sequences. Most ARGs and virulence genes were conserved across isolates originating from the three different local hospitals. Close genetic relatedness of the blaNDM-1-positive ST308 clinical and environmental isolates suggests cocirculation between the hospital environment and human hosts with the hospital environment as a potential reservoir. Core genome single nucleotide polymorphism analyses revealed possible clonal transmission of blaNDM-1-positive ST308 isolates between the three hospitals over 7 years. Bloodstream isolates accounted for six of 95 (6.3%) clinical isolates. This study reports the introduction of a pathogenic blaNDM-1-positive P. aeruginosa ST308 more than a decade ago in Singapore and warrants surveillance for wider dissemination. National Medical Research Council (NMRC) Published version This research is supported by the Singapore Ministry of Health's (MOH) National Medical Research Council (NMRC) under its NMRC Collaborative Grant: Collaborative Solutions Targeting Antimicrobial Resistance Threats in Health Systems (CoSTAR-HS) (NMRC CG21APR2005), NMRC Clinician Scientist Award (MOH-000276), and NMRC Clinician Scientist Individual Research Grant (MOH-CIRG18Nov-0034). Additional support was provided by the German Federal Ministry of Health (BMG) COVID-19 Research and Development Funding to the World Health Organization (WHO; award 70826). 2023-11-02T00:59:58Z 2023-11-02T00:59:58Z 2023 Journal Article Prakki, S. R. S., Hon, P. Y., Lim, Z. Q., Thevasagayam, N. M., Loy, S. Q. D., De, P. P., Marimuthu, K., Vasoo, S. & Ng, O. T. (2023). Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore. Microbiology Spectrum, 11(3), e0403322-. https://dx.doi.org/10.1128/spectrum.04033-22 2165-0497 https://hdl.handle.net/10356/171637 10.1128/spectrum.04033-22 37042789 2-s2.0-85163913539 3 11 e0403322 en NMRCCG21APR200 MOH-000276 MOH-CIRG18Nov-003 Microbiology Spectrum © 2023 Prakki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0International license. application/pdf |
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Science::Medicine Carbapenemase Pseudomonas Aeruginosa Prakki, Sai Rama Sridatta Hon, Pei Yun Lim, Ze Qin Thevasagayam, Natascha May Loy, Song Qi Dennis De, Partha Pratim Marimuthu, Kalisvar Vasoo, Shawn Ng, Oon Tek Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
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Pseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as blaIMP and blaVIM but has been rarely associated with blaNDM-1. A total of 199 P. aeruginosa ST308 clinical and environmental isolates obtained between April 2019 and November 2020 from a tertiary-care hospital in Singapore were characterized using whole-genome sequencing. In addition, 71 blaNDM-1-positive ST308 whole-genome sequences from two other local tertiary-care hospitals in Singapore and 83 global blaNDM-1-negative ST308 whole-genome sequences in public databases were included to assess phylogenetic relationships and perform genome analyses. Phylogenetic analysis and divergent time estimation revealed that blaNDM-1-positive P. aeruginosa ST308 was introduced into Singapore in 2005 (95 % highest posterior density: 2001 to 2008). Core genome, resistome, and analyses of all local blaNDM-1-positive ST308 isolates showed chromosomal integration of multiple antibiotic resistance genes (ARGs) [aac(3)-Id, aac(6')-Il, aadA6, aadA11, dfrB5, msr(E), floR, sul2, and qnrVC1], which was absent in global blaNDM-1-negative ST308 sequences. Most ARGs and virulence genes were conserved across isolates originating from the three different local hospitals. Close genetic relatedness of the blaNDM-1-positive ST308 clinical and environmental isolates suggests cocirculation between the hospital environment and human hosts with the hospital environment as a potential reservoir. Core genome single nucleotide polymorphism analyses revealed possible clonal transmission of blaNDM-1-positive ST308 isolates between the three hospitals over 7 years. Bloodstream isolates accounted for six of 95 (6.3%) clinical isolates. This study reports the introduction of a pathogenic blaNDM-1-positive P. aeruginosa ST308 more than a decade ago in Singapore and warrants surveillance for wider dissemination. |
author2 |
Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Prakki, Sai Rama Sridatta Hon, Pei Yun Lim, Ze Qin Thevasagayam, Natascha May Loy, Song Qi Dennis De, Partha Pratim Marimuthu, Kalisvar Vasoo, Shawn Ng, Oon Tek |
format |
Article |
author |
Prakki, Sai Rama Sridatta Hon, Pei Yun Lim, Ze Qin Thevasagayam, Natascha May Loy, Song Qi Dennis De, Partha Pratim Marimuthu, Kalisvar Vasoo, Shawn Ng, Oon Tek |
author_sort |
Prakki, Sai Rama Sridatta |
title |
Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
title_short |
Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
title_full |
Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
title_fullStr |
Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
title_full_unstemmed |
Dissemination of Pseudomonas aeruginosa blaNDM-1-positive ST308 clone in Singapore |
title_sort |
dissemination of pseudomonas aeruginosa blandm-1-positive st308 clone in singapore |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/171637 |
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1783955532892078080 |