Effects of high fat diet on intestinal toll-like receptor activity
In recent years, there has been a growing number of research on gut health, primarily due to its influence on multiple organs within our body. The gut-brain axis and gut-liver axis are well established examples of this influence. Moreover, a novel concept known as ‘leaky gut’ has been implicated as...
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sg-ntu-dr.10356-1724852023-12-18T15:33:03Z Effects of high fat diet on intestinal toll-like receptor activity Mathan Raj S/O Thayalan Tan Nguan Soon School of Biological Sciences NSTan@ntu.edu.sg Science::Biological sciences In recent years, there has been a growing number of research on gut health, primarily due to its influence on multiple organs within our body. The gut-brain axis and gut-liver axis are well established examples of this influence. Moreover, a novel concept known as ‘leaky gut’ has been implicated as a driving factor in several diseases. A recent study on the effect of diet on intestinal permeability revealed that High-Fat Diet (HFD) causes increased intestinal permeability. Toll-Like Receptors (TLRs) have been shown to have a role in maintaining gut barrier integrity. Furthermore, Angptl4 have been implicated in intestinal permeability. Therefore, this study aimed to examine how a HFD affects the expression of TLRs, specifically TLR2, TLR4, TLR5 and TLR6 in the Duodenum, Jejunum and Ileum. Additionally, it sought to determine the impact of intestinal Angptl4 knockout on the expression of these TLRs in the distinct intestinal segments. We employed Angptl4fl/fl and Angptl4vil-/- mice and conducted RT-qPCR analysis to assess changes in intestinal TLR expression levels. The findings are promising, shedding light on the relationship between HFD and expression of intestinal TLRs. Moreover, this study provides valuable insights into the role of intestinal Angptl4 on the expression of these TLRs. Bachelor of Science in Biological Sciences 2023-12-13T05:08:11Z 2023-12-13T05:08:11Z 2023 Final Year Project (FYP) Mathan Raj S/O Thayalan (2023). Effects of high fat diet on intestinal toll-like receptor activity. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/172485 https://hdl.handle.net/10356/172485 en application/pdf Nanyang Technological University |
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In recent years, there has been a growing number of research on gut health, primarily due to its influence on multiple organs within our body. The gut-brain axis and gut-liver axis are well established examples of this influence. Moreover, a novel concept known as ‘leaky gut’ has been implicated as a driving factor in several diseases. A recent study on the effect of diet on intestinal permeability revealed that High-Fat Diet (HFD) causes increased intestinal permeability. Toll-Like Receptors (TLRs) have been shown to have a role in maintaining gut barrier integrity. Furthermore, Angptl4 have been implicated in intestinal permeability. Therefore, this study aimed to examine how a HFD affects the expression of TLRs, specifically TLR2, TLR4, TLR5 and TLR6 in the Duodenum, Jejunum and Ileum. Additionally, it sought to determine the impact of intestinal Angptl4 knockout on the expression of these TLRs in the distinct intestinal segments. We employed Angptl4fl/fl and Angptl4vil-/- mice and conducted RT-qPCR analysis to assess changes in intestinal TLR expression levels. The findings are promising, shedding light on the relationship between HFD and expression of intestinal TLRs. Moreover, this study provides valuable insights into the role of intestinal Angptl4 on the expression of these TLRs. |
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Tan Nguan Soon |
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Tan Nguan Soon Mathan Raj S/O Thayalan |
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Final Year Project |
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Mathan Raj S/O Thayalan |
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Mathan Raj S/O Thayalan |
title |
Effects of high fat diet on intestinal toll-like receptor activity |
title_short |
Effects of high fat diet on intestinal toll-like receptor activity |
title_full |
Effects of high fat diet on intestinal toll-like receptor activity |
title_fullStr |
Effects of high fat diet on intestinal toll-like receptor activity |
title_full_unstemmed |
Effects of high fat diet on intestinal toll-like receptor activity |
title_sort |
effects of high fat diet on intestinal toll-like receptor activity |
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Nanyang Technological University |
publishDate |
2023 |
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https://hdl.handle.net/10356/172485 |
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1787136807712849920 |