A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues

A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues

To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not c...

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Main Authors: Adamson, Christopher, Liang, Yaquan, Feng, Shiliu, Ng, Allan Wee Ren, Qiao, Yuan
Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
Format: Article
Language:English
Published: 2024
Subjects:
Medicine, Health and Life Sciences
Ligands
Peptides
Online Access:https://hdl.handle.net/10356/174232
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1742322024-03-22T15:31:52Z A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues Adamson, Christopher Liang, Yaquan Feng, Shiliu Ng, Allan Wee Ren Qiao, Yuan School of Chemistry, Chemical Engineering and Biotechnology Medicine, Health and Life Sciences Ligands Peptides To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not confer ligand stereoselectivity; yet NAGK strictly discriminates against the disaccharide NOD2 agonists for phosphorylation in vitro, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2. Published version 2024-03-22T03:37:07Z 2024-03-22T03:37:07Z 2024 Journal Article Adamson, C., Liang, Y., Feng, S., Ng, A. W. R. & Qiao, Y. (2024). A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues. Chemical Communications, 60(16), 2212-2215. https://dx.doi.org/10.1039/d3cc05807g 1359-7345 https://hdl.handle.net/10356/174232 10.1039/d3cc05807g 38305731 2-s2.0-85183958188 16 60 2212 2215 en Chemical Communications © 2024 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Medicine, Health and Life Sciences
Ligands
Peptides
spellingShingle Medicine, Health and Life Sciences
Ligands
Peptides
Adamson, Christopher
Liang, Yaquan
Feng, Shiliu
Ng, Allan Wee Ren
Qiao, Yuan
A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
description To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not confer ligand stereoselectivity; yet NAGK strictly discriminates against the disaccharide NOD2 agonists for phosphorylation in vitro, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2.
author2 School of Chemistry, Chemical Engineering and Biotechnology
author_facet School of Chemistry, Chemical Engineering and Biotechnology
Adamson, Christopher
Liang, Yaquan
Feng, Shiliu
Ng, Allan Wee Ren
Qiao, Yuan
format Article
author Adamson, Christopher
Liang, Yaquan
Feng, Shiliu
Ng, Allan Wee Ren
Qiao, Yuan
author_sort Adamson, Christopher
title A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
title_short A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
title_full A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
title_fullStr A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
title_full_unstemmed A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
title_sort closer look at ligand specificity for cellular activation of nod2 with synthetic muramyl dipeptide analogues
publishDate 2024
url https://hdl.handle.net/10356/174232
_version_ 1794549456247455744

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