3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis

Repairing bone defects is a complex multi-stage physiological process involving the coupling of early angiogenesis and later osteogenesis and is often complicated by infection, therefore, this process remains a major clinical problem. Although functional drug-loaded engineered scaffolds are promisin...

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Main Authors: Song, Yongteng, Hu, Qingxi, Liu, Suihong, Wang, Yahao, Jia, Lijun, Hu, Xinli, Huang, Changjin, Zhang, Haiguang
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2024
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Online Access:https://hdl.handle.net/10356/180767
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1807672024-10-26T16:49:02Z 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis Song, Yongteng Hu, Qingxi Liu, Suihong Wang, Yahao Jia, Lijun Hu, Xinli Huang, Changjin Zhang, Haiguang School of Mechanical and Aerospace Engineering Engineering Large bone defect repair Vascularized bone scaffold Repairing bone defects is a complex multi-stage physiological process involving the coupling of early angiogenesis and later osteogenesis and is often complicated by infection, therefore, this process remains a major clinical problem. Although functional drug-loaded engineered scaffolds are promising for in resolving this issue, effectively replicating the natural healing cascade via sequential delivery of angiogenic and osteogenic signals remains a challenge. In this study, a vascularized bone scaffold loaded with copper ions (Cu2+) and mesoporous silica nanoparticles (MSNs) preloaded with dexamethasone (DEX) (MSNs@DEX) was fabricated via 3D printing to achieve coupled angiogenesis and osteogenesis. This scaffold promoted early angiogenesis through the rapid release of Cu2+ and later-stage osteogenesis through the gradual release of DEX, thus mirroring physiological bone repair processes. Our systematic characterization revealed that the scaffold exhibited favorable mechanical properties with a compression modulus of 25.49 ± 2.85 MPa to provide mechanical support, and had obvious antibacterial activity, confirming the sequential release of Cu2+ and DEX in vitro. Our in vitro and in vivo experiments further demonstrated that the scaffold had great biocompatibility and promoted angiogenesis and osteogenesis. Hence, our findings underscore the clinical potential of this vascularized bone scaffold for large bone defect repair. Submitted/Accepted version The authors acknowledge funding support from the National Natural Science Foundation of China (Grant Nos: 52175474, 52275498). 2024-10-23T04:42:41Z 2024-10-23T04:42:41Z 2024 Journal Article Song, Y., Hu, Q., Liu, S., Wang, Y., Jia, L., Hu, X., Huang, C. & Zhang, H. (2024). 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis. Chemical Engineering Journal, 496, 153662-. https://dx.doi.org/10.1016/j.cej.2024.153662 1385-8947 https://hdl.handle.net/10356/180767 10.1016/j.cej.2024.153662 2-s2.0-85197513301 496 153662 en Chemical Engineering Journal © 2024 Elsevier B.V. All rights reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1016/j.cej.2024.153662. application/pdf application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering
Large bone defect repair
Vascularized bone scaffold
spellingShingle Engineering
Large bone defect repair
Vascularized bone scaffold
Song, Yongteng
Hu, Qingxi
Liu, Suihong
Wang, Yahao
Jia, Lijun
Hu, Xinli
Huang, Changjin
Zhang, Haiguang
3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
description Repairing bone defects is a complex multi-stage physiological process involving the coupling of early angiogenesis and later osteogenesis and is often complicated by infection, therefore, this process remains a major clinical problem. Although functional drug-loaded engineered scaffolds are promising for in resolving this issue, effectively replicating the natural healing cascade via sequential delivery of angiogenic and osteogenic signals remains a challenge. In this study, a vascularized bone scaffold loaded with copper ions (Cu2+) and mesoporous silica nanoparticles (MSNs) preloaded with dexamethasone (DEX) (MSNs@DEX) was fabricated via 3D printing to achieve coupled angiogenesis and osteogenesis. This scaffold promoted early angiogenesis through the rapid release of Cu2+ and later-stage osteogenesis through the gradual release of DEX, thus mirroring physiological bone repair processes. Our systematic characterization revealed that the scaffold exhibited favorable mechanical properties with a compression modulus of 25.49 ± 2.85 MPa to provide mechanical support, and had obvious antibacterial activity, confirming the sequential release of Cu2+ and DEX in vitro. Our in vitro and in vivo experiments further demonstrated that the scaffold had great biocompatibility and promoted angiogenesis and osteogenesis. Hence, our findings underscore the clinical potential of this vascularized bone scaffold for large bone defect repair.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Song, Yongteng
Hu, Qingxi
Liu, Suihong
Wang, Yahao
Jia, Lijun
Hu, Xinli
Huang, Changjin
Zhang, Haiguang
format Article
author Song, Yongteng
Hu, Qingxi
Liu, Suihong
Wang, Yahao
Jia, Lijun
Hu, Xinli
Huang, Changjin
Zhang, Haiguang
author_sort Song, Yongteng
title 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
title_short 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
title_full 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
title_fullStr 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
title_full_unstemmed 3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
title_sort 3d printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis
publishDate 2024
url https://hdl.handle.net/10356/180767
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