Evidence that the SARS-CoV-2 S protein undergoes a conformational change at the Golgi complex that leads to the formation of virus neutralising antibody binding epitopes in the S1 protein subunit

• Main Authors: Wu, Yanjun, Lai, Soak Kuan, Chan, Conrad En-Zuo, Tan, Boon Huan, Sugrue, Richard J.
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2024
• Subjects: Medicine, Health and Life Sciences; SARS-CoV-2; S protein
• Online Access: https://hdl.handle.net/10356/180807
• Institution: Nanyang Technological University

Recombinant SARS-CoV-2 S protein expression was examined in Vero cells by imaging using the human monoclonal antibody panel (PD4, PD5, sc23, and sc29). The PD4 and sc29 antibodies recognised conformational specific epitopes in the S2 protein subunit at the Endoplasmic reticulum and Golgi complex. Wh...