Investigating different dynamic pHP1α states in their KCl-mediated liquid-liquid phase separation (LLPS) using solid-state NMR (SSNMR) and molecular dynamic (MD) simulations

• Main Authors: Chin, Sze Yuet, Chen, Yinglu, Zhao, Lei, Liu, Xinyi, Chng, Choon-Peng, Soman, Aghil, Nordenskiöld, Lars, Huang, Changjin, Shi, Xiangyan, Xue, Kai
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2025
• Subjects: Chemistry; Molecular docking; Nuclear magnetic resonance
• Online Access: https://hdl.handle.net/10356/182262
• Institution: Nanyang Technological University

Chromatin phase separation is dynamically regulated by many factors, such as post-translational modifications and effector proteins, and plays a critical role in genomic activities. The liquid-liquid phase separation (LLPS) of chromatin and/or effector proteins has been observed both in vitro and in...