Detection of variant of hepatitis B virus and beta-globin through DNA technology

• Main Author: Chia, Chee Poh.
• Other Authors: Gan Yap, Yik Yuen
• Format: Theses and Dissertations
• Language: English
• Published: 2009
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Virology
• Online Access: http://hdl.handle.net/10356/20362
• Institution: Nanyang Technological University

Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radio...