Detection of variant of hepatitis B virus and beta-globin through DNA technology

Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radio...

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Main Author: Chia, Chee Poh.
Other Authors: Gan Yap, Yik Yuen
Format: Theses and Dissertations
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/20362
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-203622020-11-01T06:18:04Z Detection of variant of hepatitis B virus and beta-globin through DNA technology Chia, Chee Poh. Gan Yap, Yik Yuen National Institute of Education DRNTU::Science::Biological sciences::Microbiology::Virology Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radioactive direct DNA cycle sequencing. Master of Science 2009-12-14T09:36:35Z 2009-12-14T09:36:35Z 1996 1996 Thesis http://hdl.handle.net/10356/20362 en NANYANG TECHNOLOGICAL UNIVERSITY 153 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Virology
Chia, Chee Poh.
Detection of variant of hepatitis B virus and beta-globin through DNA technology
description Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radioactive direct DNA cycle sequencing.
author2 Gan Yap, Yik Yuen
author_facet Gan Yap, Yik Yuen
Chia, Chee Poh.
format Theses and Dissertations
author Chia, Chee Poh.
author_sort Chia, Chee Poh.
title Detection of variant of hepatitis B virus and beta-globin through DNA technology
title_short Detection of variant of hepatitis B virus and beta-globin through DNA technology
title_full Detection of variant of hepatitis B virus and beta-globin through DNA technology
title_fullStr Detection of variant of hepatitis B virus and beta-globin through DNA technology
title_full_unstemmed Detection of variant of hepatitis B virus and beta-globin through DNA technology
title_sort detection of variant of hepatitis b virus and beta-globin through dna technology
publishDate 2009
url http://hdl.handle.net/10356/20362
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