Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins

211 p.

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Bibliographic Details
Main Author:	Lu, Yi Wei
Other Authors:	Chen Wei Ning, William
Format:	Theses and Dissertations
Published:	2010
Subjects:	DRNTU::Science::Biological sciences::Microbiology::Virology
Online Access:	https://hdl.handle.net/10356/35759
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Institution:	Nanyang Technological University

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spelling	sg-ntu-dr.10356-357592023-02-28T18:41:18Z Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins Lu, Yi Wei Chen Wei Ning, William School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology 211 p. The smallest protein of hepatitis B virus (HBV), HBx, has been implicated in the development of liver diseases by interfering with normal cellular progresses. Its role in cell proliferation has been unclear as both pro-apoptotic and anti-apoptotic activities have been reported. We showed molecular evidence that HBx induced apoptosis in HepG2 cells. ABcl-2 Homology Domain 3 (BH3) was identified in HBx, which interacted with anti-apoptotic but not pro-apoptotic members of the Bcl-2 family of proteins. HBx induced apoptosis when transfected into HepG2 cells, as demonstrated by both flow cytometry and caspase-3 activity. However, HBx protein may not be stable in apoptotic cells triggered by its own expression as only its mRNA or the fusion protein with the glutathione-S-transferase was detected in transfected cells. These results suggested that HBx behaved as a pro-apoptotic protein and was able to induce apoptosis. DOCTOR OF PHILOSOPHY (SBS) 2010-04-23T01:38:04Z 2010-04-23T01:38:04Z 2007 2007 Thesis Lu, Y. W. (2007). Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/35759 10.32657/10356/35759 application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
topic	DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle	DRNTU::Science::Biological sciences::Microbiology::Virology Lu, Yi Wei Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
description	211 p.
author2	Chen Wei Ning, William
author_facet	Chen Wei Ning, William Lu, Yi Wei
format	Theses and Dissertations
author	Lu, Yi Wei
author_sort	Lu, Yi Wei
title	Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
title_short	Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
title_full	Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
title_fullStr	Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
title_full_unstemmed	Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins
title_sort	characterization of apoptosis induced by bh3-containing hepatitis b virus proteins
publishDate	2010
url	https://hdl.handle.net/10356/35759
_version_	1759855717922111488

Characterization of apoptosis induced by BH3-containing hepatitis B virus proteins

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