Functional characterization of the beta2 integrins
192 p.
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2010
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| Online Access: | https://hdl.handle.net/10356/35762 |
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sg-ntu-dr.10356-357622023-02-28T18:36:21Z Functional characterization of the beta2 integrins Tang, Ren Hong Law Sai Kit, Alex Tan Suet Mien School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology 192 p. aLp2 (CD 1 la/CD 18, LFA-1), aMp2 (CD1 lb/CD 18, Mac-1) and, aXp2 (CD1 lc/CD18, pi50, 95) are members of P2 integrins involved in leukocyte function during immune and inflammatory responses. The first part of this study, the epitope of a p2-specific antibody MEM 148 was fine mapped to the hybrid domain and it is dependent on the movement of the hybrid domain upon activation. Furthermore, we made use of the activation-reporter property of MEM 148 to analyze the different aLp2 affinity states required for ICAM-1 and ICAM-3 binding. Unlike ICAM-1 binding, which required only one activating agent, aLp2/ICAM-3 binding required both Mg/EGTA and an activating mAb. This suggests that aLp2 with intermediate affinity is sufficient to bind ICAM-1 but not ICAM-3, which requires a high affinity state. DOCTOR OF PHILOSOPHY (SBS) 2010-04-23T01:38:15Z 2010-04-23T01:38:15Z 2007 2007 Thesis Tang, R. H. (2007). Functional characterization of the beta2 integrins. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/35762 10.32657/10356/35762 application/pdf |
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DRNTU::Science::Biological sciences::Molecular biology |
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DRNTU::Science::Biological sciences::Molecular biology Tang, Ren Hong Functional characterization of the beta2 integrins |
| description |
192 p. |
| author2 |
Law Sai Kit, Alex |
| author_facet |
Law Sai Kit, Alex Tang, Ren Hong |
| format |
Theses and Dissertations |
| author |
Tang, Ren Hong |
| author_sort |
Tang, Ren Hong |
| title |
Functional characterization of the beta2 integrins |
| title_short |
Functional characterization of the beta2 integrins |
| title_full |
Functional characterization of the beta2 integrins |
| title_fullStr |
Functional characterization of the beta2 integrins |
| title_full_unstemmed |
Functional characterization of the beta2 integrins |
| title_sort |
functional characterization of the beta2 integrins |
| publishDate |
2010 |
| url |
https://hdl.handle.net/10356/35762 |
| _version_ |
1759854404725374976 |