Protein loading and delivery : comparison between mesostructured and non porous bioceramics
This report aims to compare two different classes of bioceramics in protein loading and release for mesostructured and non porous bioceramics. Different types of protein and their concentrations were studied in relative to the loading efficiency of the various bioceramics. Bioactive glass (BG 90S...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/36170 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | This report aims to compare two different classes of bioceramics in protein
loading and release for mesostructured and non porous bioceramics. Different
types of protein and their concentrations were studied in relative to the loading
efficiency of the various bioceramics. Bioactive glass (BG 90S), mesoporous
bioactive glasses (MBG 90S) and mesoporous hydroxyapatite (MHA) were
prepared using nonionic block copolymer surfactants (F127) as structuredirecting
agent. Hydroxyapatite (HA) was commercially purchased as a part of
the comparison. Different concentrations of Bovine Serum Albumin (BSA) and
Lysozyme Chlorine crystalline (from egg white) were encapsulated into MBG
90S, BG 90S, MHA and HA by adsorption method in water and in vitro release
were conducted in Phosphate Buffered Solution (PBS). The experimental results
were characterized using thermo gravitmetric analysis (TGA), UV-vis
spectrophotometry and nitrogen adsorption analysis (BET). For bioceramics
loaded with lysozyme, the results demonstrated that the protein loading
efficiency of MBGs and MHA were significantly higher than that of BGs and HAs
for different lysozyme concentrations. Increasing the initial concentration of
lysozyme loaded into the bioceramics will also lead to an increase in loading
efficiency. For bioceramics loaded with Bovine Sereum Albumin, the results
demonstrated that the protein loading efficiency of MHA were significantly higher
than that of HA for different BSA concentrations. Increasing the initial
concentration of BSA loaded into the bioceramics will lead to a decrease in the
loading efficiency. Generally, pore size, surface area of the bioceramics as well
as the initial loaded protein concentration are generally the dominant factors in
controlling the efficiency of protein loading into bioceramics |
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