Protein loading and delivery : comparison between mesostructured and non porous bioceramics

This report aims to compare two different classes of bioceramics in protein loading and release for mesostructured and non porous bioceramics. Different types of protein and their concentrations were studied in relative to the loading efficiency of the various bioceramics. Bioactive glass (BG 90S...

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Main Author: Teo, Kenvin Shi Yu.
Other Authors: Loo Say Chye Joachim
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/36170
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-361702023-03-04T15:32:57Z Protein loading and delivery : comparison between mesostructured and non porous bioceramics Teo, Kenvin Shi Yu. Loo Say Chye Joachim School of Materials Science and Engineering Ng Su Xiu DRNTU::Engineering::Materials::Biomaterials This report aims to compare two different classes of bioceramics in protein loading and release for mesostructured and non porous bioceramics. Different types of protein and their concentrations were studied in relative to the loading efficiency of the various bioceramics. Bioactive glass (BG 90S), mesoporous bioactive glasses (MBG 90S) and mesoporous hydroxyapatite (MHA) were prepared using nonionic block copolymer surfactants (F127) as structuredirecting agent. Hydroxyapatite (HA) was commercially purchased as a part of the comparison. Different concentrations of Bovine Serum Albumin (BSA) and Lysozyme Chlorine crystalline (from egg white) were encapsulated into MBG 90S, BG 90S, MHA and HA by adsorption method in water and in vitro release were conducted in Phosphate Buffered Solution (PBS). The experimental results were characterized using thermo gravitmetric analysis (TGA), UV-vis spectrophotometry and nitrogen adsorption analysis (BET). For bioceramics loaded with lysozyme, the results demonstrated that the protein loading efficiency of MBGs and MHA were significantly higher than that of BGs and HAs for different lysozyme concentrations. Increasing the initial concentration of lysozyme loaded into the bioceramics will also lead to an increase in loading efficiency. For bioceramics loaded with Bovine Sereum Albumin, the results demonstrated that the protein loading efficiency of MHA were significantly higher than that of HA for different BSA concentrations. Increasing the initial concentration of BSA loaded into the bioceramics will lead to a decrease in the loading efficiency. Generally, pore size, surface area of the bioceramics as well as the initial loaded protein concentration are generally the dominant factors in controlling the efficiency of protein loading into bioceramics Bachelor of Engineering (Materials Engineering) 2010-04-23T03:27:25Z 2010-04-23T03:27:25Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/36170 en Nanyang Technological University 48 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Teo, Kenvin Shi Yu.
Protein loading and delivery : comparison between mesostructured and non porous bioceramics
description This report aims to compare two different classes of bioceramics in protein loading and release for mesostructured and non porous bioceramics. Different types of protein and their concentrations were studied in relative to the loading efficiency of the various bioceramics. Bioactive glass (BG 90S), mesoporous bioactive glasses (MBG 90S) and mesoporous hydroxyapatite (MHA) were prepared using nonionic block copolymer surfactants (F127) as structuredirecting agent. Hydroxyapatite (HA) was commercially purchased as a part of the comparison. Different concentrations of Bovine Serum Albumin (BSA) and Lysozyme Chlorine crystalline (from egg white) were encapsulated into MBG 90S, BG 90S, MHA and HA by adsorption method in water and in vitro release were conducted in Phosphate Buffered Solution (PBS). The experimental results were characterized using thermo gravitmetric analysis (TGA), UV-vis spectrophotometry and nitrogen adsorption analysis (BET). For bioceramics loaded with lysozyme, the results demonstrated that the protein loading efficiency of MBGs and MHA were significantly higher than that of BGs and HAs for different lysozyme concentrations. Increasing the initial concentration of lysozyme loaded into the bioceramics will also lead to an increase in loading efficiency. For bioceramics loaded with Bovine Sereum Albumin, the results demonstrated that the protein loading efficiency of MHA were significantly higher than that of HA for different BSA concentrations. Increasing the initial concentration of BSA loaded into the bioceramics will lead to a decrease in the loading efficiency. Generally, pore size, surface area of the bioceramics as well as the initial loaded protein concentration are generally the dominant factors in controlling the efficiency of protein loading into bioceramics
author2 Loo Say Chye Joachim
author_facet Loo Say Chye Joachim
Teo, Kenvin Shi Yu.
format Final Year Project
author Teo, Kenvin Shi Yu.
author_sort Teo, Kenvin Shi Yu.
title Protein loading and delivery : comparison between mesostructured and non porous bioceramics
title_short Protein loading and delivery : comparison between mesostructured and non porous bioceramics
title_full Protein loading and delivery : comparison between mesostructured and non porous bioceramics
title_fullStr Protein loading and delivery : comparison between mesostructured and non porous bioceramics
title_full_unstemmed Protein loading and delivery : comparison between mesostructured and non porous bioceramics
title_sort protein loading and delivery : comparison between mesostructured and non porous bioceramics
publishDate 2010
url http://hdl.handle.net/10356/36170
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