Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.

Dopamine (DA) plays a critical role in cognition and reward. Therefore, dysregulation of DA neurotransmission has been implicated in neurological diseases such as Parkinson’s disease, and psychiatric disorders such as attention-deficit hyperactive disorder. Tyrosine hydroxylase (TH) is the rate-limi...

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Main Author: Lim, Gerard Shing-Wan.
Other Authors: Zhang Xiaodong
Format: Final Year Project
Language:English
Published: 2010
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Online Access:http://hdl.handle.net/10356/38733
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-387332023-02-28T18:00:04Z Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter. Lim, Gerard Shing-Wan. Zhang Xiaodong School of Biological Sciences Duke-NUS Medical School DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Dopamine (DA) plays a critical role in cognition and reward. Therefore, dysregulation of DA neurotransmission has been implicated in neurological diseases such as Parkinson’s disease, and psychiatric disorders such as attention-deficit hyperactive disorder. Tyrosine hydroxylase (TH) is the rate-limiting enzyme in DA synthesis and its regulation has been found to be altered in transgenic mice lacking the dopamine transporter (DAT). DAT plays a critical role in the re-uptake of DA release in the synaptic cleft, thus determining the intensity and duration of DA neurotransmission. In this study, we further explored age-dependent regulation of TH in DAT knockout (KO) mice. The DAT mice exhibited a state of hyperactivity in our 10-minute locomotor activity test, a behavioral characteristic consistent in previous studies of such mice. It was found in our research that there were no significant differences in TH and pTH levels between WT and DAT KO mice up to 3 week-old. However, a dramatic and gradual decrease of both TH and pTH levels were observed from 4 week-old to 16 week-old mice. Thus, our findings provide further evidence that DAT not only plays a role in controlling the duration of DA neurotransmission, but also in the regulation of presynaptic TH homeostasis. Bachelor of Science in Biological Sciences 2010-05-18T02:14:16Z 2010-05-18T02:14:16Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/38733 en Nanyang Technological University 33 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology
spellingShingle DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology
Lim, Gerard Shing-Wan.
Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
description Dopamine (DA) plays a critical role in cognition and reward. Therefore, dysregulation of DA neurotransmission has been implicated in neurological diseases such as Parkinson’s disease, and psychiatric disorders such as attention-deficit hyperactive disorder. Tyrosine hydroxylase (TH) is the rate-limiting enzyme in DA synthesis and its regulation has been found to be altered in transgenic mice lacking the dopamine transporter (DAT). DAT plays a critical role in the re-uptake of DA release in the synaptic cleft, thus determining the intensity and duration of DA neurotransmission. In this study, we further explored age-dependent regulation of TH in DAT knockout (KO) mice. The DAT mice exhibited a state of hyperactivity in our 10-minute locomotor activity test, a behavioral characteristic consistent in previous studies of such mice. It was found in our research that there were no significant differences in TH and pTH levels between WT and DAT KO mice up to 3 week-old. However, a dramatic and gradual decrease of both TH and pTH levels were observed from 4 week-old to 16 week-old mice. Thus, our findings provide further evidence that DAT not only plays a role in controlling the duration of DA neurotransmission, but also in the regulation of presynaptic TH homeostasis.
author2 Zhang Xiaodong
author_facet Zhang Xiaodong
Lim, Gerard Shing-Wan.
format Final Year Project
author Lim, Gerard Shing-Wan.
author_sort Lim, Gerard Shing-Wan.
title Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
title_short Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
title_full Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
title_fullStr Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
title_full_unstemmed Exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
title_sort exploring age-dependent regulation of tyrosine hydroxylase in mice lacking the dopamine transporter.
publishDate 2010
url http://hdl.handle.net/10356/38733
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