Generation of lentiviral-mediated shRNA vectors to inhibit the expression of caspase-1 and Pycard/ASC in dendritic cells.

• Main Author: Tan, Bee Kim.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Immunology
• Online Access: http://hdl.handle.net/10356/39450
• Institution: Nanyang Technological University

Dendritic cells (DC) originate from hematopoietic precursors and they detect endogenous and exogenous stimuli which lead to an inflammatory response. The “inflammasome” is a large protein complex which consists of Caspase-1 and Pycard/ASC which are involved in IL-1β and IL-18 processing. However, th...