Mapping protein-protein interactions in the apoptosis pathway.

Apoptosis or programmed cell death is a crucial process in almost all living organisms. It is important for evolution, homeostatic maintenance and proper development of an organism. Many apoptosis-related studies had been conducted using genetic, cellular, systematical, and structural biological app...

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Main Author: Triani, Renata.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/39609
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-396092023-02-28T18:01:16Z Mapping protein-protein interactions in the apoptosis pathway. Triani, Renata. School of Biological Sciences A*STAR Biopolis M.S. Madhusudhan DRNTU::Science::Biological sciences Apoptosis or programmed cell death is a crucial process in almost all living organisms. It is important for evolution, homeostatic maintenance and proper development of an organism. Many apoptosis-related studies had been conducted using genetic, cellular, systematical, and structural biological approaches. These studies have resulted in the identification of hundreds of proteins known to regulate the apoptosis process and hence increased the understanding of its mechanism. My project focuses on modeling the three-dimensional structures of protein complexes that are formed during apoptosis in Drosophila melanogaster. By building three-dimensional model of apoptotic proteins, we can identify what proteins interact, how they interact and what residues are important for these interactions. To achieve this, Drosophila melanogaster apoptotic proteins were structurally annotated for known protein binding surfaces using the interface library developed by Dr. Minh Ngoc Nguyen. Proteins with complementary interfaces were matched structurally to model the interaction between proteins in three-dimensions and several assessments were performed to refine the models of complex structure. For the final assessment, a statistical potential was performed to define the true interactions. In this project, 38 new interactions between 27 proteins in the apoptosis pathway of Drosophila melanogaster were identified. These candidate complexes will then be validated experimentally. Bachelor of Science in Biological Sciences 2010-06-01T07:06:20Z 2010-06-01T07:06:20Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39609 en Nanyang Technological University 45 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Triani, Renata.
Mapping protein-protein interactions in the apoptosis pathway.
description Apoptosis or programmed cell death is a crucial process in almost all living organisms. It is important for evolution, homeostatic maintenance and proper development of an organism. Many apoptosis-related studies had been conducted using genetic, cellular, systematical, and structural biological approaches. These studies have resulted in the identification of hundreds of proteins known to regulate the apoptosis process and hence increased the understanding of its mechanism. My project focuses on modeling the three-dimensional structures of protein complexes that are formed during apoptosis in Drosophila melanogaster. By building three-dimensional model of apoptotic proteins, we can identify what proteins interact, how they interact and what residues are important for these interactions. To achieve this, Drosophila melanogaster apoptotic proteins were structurally annotated for known protein binding surfaces using the interface library developed by Dr. Minh Ngoc Nguyen. Proteins with complementary interfaces were matched structurally to model the interaction between proteins in three-dimensions and several assessments were performed to refine the models of complex structure. For the final assessment, a statistical potential was performed to define the true interactions. In this project, 38 new interactions between 27 proteins in the apoptosis pathway of Drosophila melanogaster were identified. These candidate complexes will then be validated experimentally.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Triani, Renata.
format Final Year Project
author Triani, Renata.
author_sort Triani, Renata.
title Mapping protein-protein interactions in the apoptosis pathway.
title_short Mapping protein-protein interactions in the apoptosis pathway.
title_full Mapping protein-protein interactions in the apoptosis pathway.
title_fullStr Mapping protein-protein interactions in the apoptosis pathway.
title_full_unstemmed Mapping protein-protein interactions in the apoptosis pathway.
title_sort mapping protein-protein interactions in the apoptosis pathway.
publishDate 2010
url http://hdl.handle.net/10356/39609
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