Sequence specific colorimetric detection of DNA post-PCR using split DNAzyme

• Main Author: Aw, Darius Kang Lie
• Other Authors: Poh Chueh Loo
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/39817
• Institution: Nanyang Technological University

The split G-quadruplex DNAzyme has become a valuable tool for visual detection. Most reports on its use have however been constrained to proof-of-concept demonstrations using synthetic oligonucleotides. This is due to the inherent complexities of the assay, and the multiple components involved. H...