Optimization of tobacco etch virus (TEV) protease cleavage in fusion proteins.

• Main Author: Yaohari, Hazarki.
• Other Authors: Susanna Leong Su Jan
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnological production
• Online Access: http://hdl.handle.net/10356/39819
• Institution: Nanyang Technological University

This report summarizes the study of tobacco etch virus protease (TEVp) cleavage efficiency to enhance the productivity of antimicrobial peptide human beta-defensin (HBD) 25, a promising new class of antibiotics. Four parameters were investigated: salt concentration, substrate to enzyme ratio, cleava...