Fast and efficient chemical ligation of oligonucleotides using diels-alder reaction.

• Main Author: Cheong, Vee Vee.
• Other Authors: School of Physical and Mathematical Sciences
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Science::Chemistry::Biochemistry
• Online Access: http://hdl.handle.net/10356/39857
• Institution: Nanyang Technological University

Diels-Alder chemical ligation was applied to the ligation of two and three oligonucleotides to form a single, continuous strand with an unnatural DNA backbone at the ligation site. The reaction occurred between a maleimide-labelled oligonucleotide and a furan-labelled oligonucleotide to form two ty...