Optimization of gene expression analysis in engineered tissue : determination of proper reference gene for quantitative real-time PCR in 3-d scaffold systems.
Gene expression study is widely used to obtain information of the cells activities and phenotypes. To quantify gene expression, measurement of the mRNA copy number is commonly done by qualitative RT-PCR. However, reference gene is needed to normalize the expression level of genes of interest (GOI)....
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2010
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| Online Access: | http://hdl.handle.net/10356/39966 |
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| Institution: | Nanyang Technological University |
| Language: | English |