Optimization of gene expression analysis in engineered tissue : determination of proper reference gene for quantitative real-time PCR in 3-d scaffold systems.

Gene expression study is widely used to obtain information of the cells activities and phenotypes. To quantify gene expression, measurement of the mRNA copy number is commonly done by qualitative RT-PCR. However, reference gene is needed to normalize the expression level of genes of interest (GOI)....

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Bibliographic Details
Main Author: Chooi, Wai Hon.
Other Authors: Wang Dongan
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/39966
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Institution: Nanyang Technological University
Language: English

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