New multipurpose M13Ke phage display vector for screening and developing biosensors

• Main Author: Fang, Yue Qin
• Other Authors: Brendan Patrick Orner
• Format: Theses and Dissertations
• Language: English
• Published: 2010
• Subjects: DRNTU::Science::Chemistry::Biochemistry
• Online Access: http://hdl.handle.net/10356/41574
• Institution: Nanyang Technological University

We aim to engineer a multipurpose M13KE phage display vectors which contains an additional gene III for peptide or protein display to create biosensors for glucose blood sugar levels to help fight diabetes. A truncated gene III was cloned and a fragment containing a lac/tac promoter and the gene III signal peptide sequence was assembled using overlapping-extension polymerase chain reaction. The fragments were joined using PCR via their overlapping regions. Expression analysis with anti-M13 pIII monoclonal antibody revealed their recombinant gene III expression is independent on inducer concentration, but has a temporal variation.