Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis
Articular hyaline cartilage is an avascular, aneural and alymphatic tissue that has a very limited capability to self-regenerate after damaged by injuries or degenerative diseases. This project aims to develop engineered articular cartilage for cartilage repair. We found that SMSCs infected with Ad-...
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sg-ntu-dr.10356-425122023-03-03T15:58:09Z Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis Yao, Yongchang Wang Dongan School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Articular hyaline cartilage is an avascular, aneural and alymphatic tissue that has a very limited capability to self-regenerate after damaged by injuries or degenerative diseases. This project aims to develop engineered articular cartilage for cartilage repair. We found that SMSCs infected with Ad-TGF β3 did promote the chondrogenesis of SMSCS effectively but stimulated the fibrosis. Hence, two approaches for combinational transfer of transgene TGF-β3 and anti-Col I shRNA via adenoviral vector were proposed and their effects for chondrogenesis were evaluated. Additionally, we expanded the study to another conventional cell source---chondrocytes, hypothesizing that this combinational delivery would also help chondrocytes maintain their phenotypes even after repeated monolayer passages. The results demonstrate that the combinational delivery system is effective on both inducing Col I-free chondrogenesis of SMSCs and promoting redifferentiation of dedifferentiated chondrocytes. The dual functions applied in engineered cartilage will make cell-based therapeutics more feasible and reliable for cartilage regeneration. DOCTOR OF PHILOSOPHY (SCBE) 2010-12-29T08:01:17Z 2010-12-29T08:01:17Z 2010 2010 Thesis Yao, Y. C. (2010). Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/42512 10.32657/10356/42512 en 163 p. application/pdf |
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DRNTU::Engineering::Bioengineering Yao, Yongchang Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
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Articular hyaline cartilage is an avascular, aneural and alymphatic tissue that has a very limited capability to self-regenerate after damaged by injuries or degenerative diseases. This project aims to develop engineered articular cartilage for cartilage repair. We found that SMSCs infected with Ad-TGF β3 did promote the chondrogenesis of SMSCS effectively but stimulated the fibrosis. Hence, two approaches for combinational transfer of transgene TGF-β3 and anti-Col I shRNA via adenoviral vector were proposed and their effects for chondrogenesis were evaluated. Additionally, we expanded the study to another conventional cell source---chondrocytes, hypothesizing that this combinational delivery would also help chondrocytes maintain their phenotypes even after repeated monolayer passages. The results demonstrate that the combinational delivery system is effective on both inducing Col I-free chondrogenesis of SMSCs and promoting redifferentiation of dedifferentiated chondrocytes. The dual functions applied in engineered cartilage will make cell-based therapeutics more feasible and reliable for cartilage regeneration. |
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Wang Dongan |
author_facet |
Wang Dongan Yao, Yongchang |
format |
Theses and Dissertations |
author |
Yao, Yongchang |
author_sort |
Yao, Yongchang |
title |
Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
title_short |
Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
title_full |
Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
title_fullStr |
Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
title_full_unstemmed |
Construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
title_sort |
construction and application of bi-functional adenoviral vectors for engineered articular chondrogenesis |
publishDate |
2010 |
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https://hdl.handle.net/10356/42512 |
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1759853685726248960 |