Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.

Fluorescence in situ hybridization (FISH) is a useful tool in diagnosing multiple myeloma (MM), a disease involving abnormal clones of plasma cells. In a standard FISH procedure, all types of cells within the sample are analyzed and scored. A new method, cytoplasmic immunoglobulin (cIg) FISH, has re...

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Main Author: Choo, Natasha Swee Lian.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/44856
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-448562023-02-28T18:02:09Z Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma. Choo, Natasha Swee Lian. School of Biological Sciences Singapore General Hospital Lim Soon Tiong Alvin DRNTU::Science::Biological sciences Fluorescence in situ hybridization (FISH) is a useful tool in diagnosing multiple myeloma (MM), a disease involving abnormal clones of plasma cells. In a standard FISH procedure, all types of cells within the sample are analyzed and scored. A new method, cytoplasmic immunoglobulin (cIg) FISH, has recently been developed to specifically label the plasma cells by targeting the immunoglobulin light chains. This method allows the direct analysis and scoring of the plasma cells to give a more accurate result. If it is shown to reflect a more accurate result, it may be considered to be utilized in a routine laboratory. In this project, cIg FISH was carried out on 30 fixed bone marrow MM samples that had karyotyping and standard FISH done previously and results were compared. It was observed that cIg FISH enhanced the detection rate by an overall 16.7% for 1q21 amplifications and 3.4% for 15q22 amplification. The cIg procedure was also able to pick up abnormalities that standard FISH did not. This study showed that cIg FISH is an accurate and a more sensitive assay than standard FISH. However, optimization of this time-consuming procedure is required before cIg FISH can be considered for routine diagnostic application. Bachelor of Science in Biological Sciences 2011-06-06T06:26:52Z 2011-06-06T06:26:52Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44856 en Nanyang Technological University 32 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Choo, Natasha Swee Lian.
Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
description Fluorescence in situ hybridization (FISH) is a useful tool in diagnosing multiple myeloma (MM), a disease involving abnormal clones of plasma cells. In a standard FISH procedure, all types of cells within the sample are analyzed and scored. A new method, cytoplasmic immunoglobulin (cIg) FISH, has recently been developed to specifically label the plasma cells by targeting the immunoglobulin light chains. This method allows the direct analysis and scoring of the plasma cells to give a more accurate result. If it is shown to reflect a more accurate result, it may be considered to be utilized in a routine laboratory. In this project, cIg FISH was carried out on 30 fixed bone marrow MM samples that had karyotyping and standard FISH done previously and results were compared. It was observed that cIg FISH enhanced the detection rate by an overall 16.7% for 1q21 amplifications and 3.4% for 15q22 amplification. The cIg procedure was also able to pick up abnormalities that standard FISH did not. This study showed that cIg FISH is an accurate and a more sensitive assay than standard FISH. However, optimization of this time-consuming procedure is required before cIg FISH can be considered for routine diagnostic application.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Choo, Natasha Swee Lian.
format Final Year Project
author Choo, Natasha Swee Lian.
author_sort Choo, Natasha Swee Lian.
title Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
title_short Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
title_full Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
title_fullStr Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
title_full_unstemmed Validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
title_sort validating a new plasma cell labeling technique for targeted fluorescence in situ hybridization assay in patients diagnosed with multiple myeloma.
publishDate 2011
url http://hdl.handle.net/10356/44856
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