Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer.
To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7, we had demonstrated...
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sg-ntu-dr.10356-451112023-02-28T18:04:10Z Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. Png, Jasmine Jie Min. Edwin Chong Wing Cheung School of Biological Sciences Agency for Science, Technology and Research (A*STAR) DRNTU::Science::Biological sciences::Biochemistry To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7, we had demonstrated that AP-2γ is not involved in cellular proliferation. Instead, it promotes breast cancer by mediating cell survival. Furthermore, we showed that AP-2γ silencing resulted in the down-regulation of ERα-upregulated genes, and this was verified by our over- expression experiments in U2OS Tet-on-ERα, a cell line that lacks AP-2γ expression. Hence, it can be ascertained that AP-2γ is required for the transcription of ERα-regulated genes. However, we observed a minimal effect on promoting cell survival after over-expression of AP-2γ in U2OS Tet-on-ERα. Such phenomenon could be explained by the requirement of other transcription factors that might cooperate with AP-2γ in the reprogramming machinery. Despite the partial reprogramming ability, we noticed a significant reduction in cell death in EtOH-treated U2OS Tet-on-ERα. Therefore, we can suggest that AP-2γ is able to reprogram the ERα transcription network to a certain extent. Bachelor of Science in Biological Sciences 2011-06-09T03:17:12Z 2011-06-09T03:17:12Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45111 en Nanyang Technological University 33 p. application/pdf |
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DRNTU::Science::Biological sciences::Biochemistry Png, Jasmine Jie Min. Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
description |
To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on
examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to
reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7,
we had demonstrated that AP-2γ is not involved in cellular proliferation. Instead, it promotes
breast cancer by mediating cell survival. Furthermore, we showed that AP-2γ silencing
resulted in the down-regulation of ERα-upregulated genes, and this was verified by our over-
expression experiments in U2OS Tet-on-ERα, a cell line that lacks AP-2γ expression. Hence,
it can be ascertained that AP-2γ is required for the transcription of ERα-regulated genes.
However, we observed a minimal effect on promoting cell survival after over-expression of
AP-2γ in U2OS Tet-on-ERα. Such phenomenon could be explained by the requirement of
other transcription factors that might cooperate with AP-2γ in the reprogramming machinery.
Despite the partial reprogramming ability, we noticed a significant reduction in cell death in
EtOH-treated U2OS Tet-on-ERα. Therefore, we can suggest that AP-2γ is able to reprogram
the ERα transcription network to a certain extent. |
author2 |
Edwin Chong Wing Cheung |
author_facet |
Edwin Chong Wing Cheung Png, Jasmine Jie Min. |
format |
Final Year Project |
author |
Png, Jasmine Jie Min. |
author_sort |
Png, Jasmine Jie Min. |
title |
Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
title_short |
Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
title_full |
Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
title_fullStr |
Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
title_full_unstemmed |
Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
title_sort |
functional analysis of ap-2γ as an estrogen receptor α (erα) cofactor in breast cancer. |
publishDate |
2011 |
url |
http://hdl.handle.net/10356/45111 |
_version_ |
1759853613028474880 |