Roles of mitochondria in cancer targeting therapeutics.

Dysfunctions of mitochondria have been implicated as the important factor of a wide variety of diseases. Significantly, mitochondrion has emerged as the potential targets for cancer targeting therapeutics. In particular, recent findings show that reversible protein phosphorylation, which is a crucia...

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Main Author: Sim, Kae Hwan.
Other Authors: Sze Siu Kwan
Format: Final Year Project
Language:English
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/10356/45273
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-452732023-02-28T18:03:40Z Roles of mitochondria in cancer targeting therapeutics. Sim, Kae Hwan. Sze Siu Kwan School of Biological Sciences DRNTU::Science::Biological sciences::Cytology Dysfunctions of mitochondria have been implicated as the important factor of a wide variety of diseases. Significantly, mitochondrion has emerged as the potential targets for cancer targeting therapeutics. In particular, recent findings show that reversible protein phosphorylation, which is a crucial but largely overlooked means of mitochondrial post translational modification, regulates the mitochondrial functions in the oncogenic signaling network. Comprehensive understanding of mitochondrial phosphoproteomics will thus help in breakthrough of cancer therapy. Nonetheless the substoichiometric level of protein phosphorylation largely hinders the global analysis of mitochondrial phosphoproteome. Here we report optimization of several phosphopeptide enrichment approaches including batchwise IMAC, TiO2, ERLIC, SCX and ERLIC chromatography for efficient identification of phosphopeptides and phosphoproteins. A novel combinatorial enrichment strategy that couples SDS-PAGE to batchwise ERLIC, IMAC and TiO2 sequentially has been developed to enrich for phosphopeptides of SNU1 and SNU5 cancer cells mitochondria. Finally we identified 403 and 356 phosphoproteins in SNU1 and SNU5 mitochondria respectively. A list of 44 important phosphoproteins is summarized. These results revealed diverse crucial phosphorylation activities involved in the regulation of mitochondrial functions in cancer cells. Bachelor of Science in Biological Sciences 2011-06-10T06:41:24Z 2011-06-10T06:41:24Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45273 en Nanyang Technological University 48 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Cytology
spellingShingle DRNTU::Science::Biological sciences::Cytology
Sim, Kae Hwan.
Roles of mitochondria in cancer targeting therapeutics.
description Dysfunctions of mitochondria have been implicated as the important factor of a wide variety of diseases. Significantly, mitochondrion has emerged as the potential targets for cancer targeting therapeutics. In particular, recent findings show that reversible protein phosphorylation, which is a crucial but largely overlooked means of mitochondrial post translational modification, regulates the mitochondrial functions in the oncogenic signaling network. Comprehensive understanding of mitochondrial phosphoproteomics will thus help in breakthrough of cancer therapy. Nonetheless the substoichiometric level of protein phosphorylation largely hinders the global analysis of mitochondrial phosphoproteome. Here we report optimization of several phosphopeptide enrichment approaches including batchwise IMAC, TiO2, ERLIC, SCX and ERLIC chromatography for efficient identification of phosphopeptides and phosphoproteins. A novel combinatorial enrichment strategy that couples SDS-PAGE to batchwise ERLIC, IMAC and TiO2 sequentially has been developed to enrich for phosphopeptides of SNU1 and SNU5 cancer cells mitochondria. Finally we identified 403 and 356 phosphoproteins in SNU1 and SNU5 mitochondria respectively. A list of 44 important phosphoproteins is summarized. These results revealed diverse crucial phosphorylation activities involved in the regulation of mitochondrial functions in cancer cells.
author2 Sze Siu Kwan
author_facet Sze Siu Kwan
Sim, Kae Hwan.
format Final Year Project
author Sim, Kae Hwan.
author_sort Sim, Kae Hwan.
title Roles of mitochondria in cancer targeting therapeutics.
title_short Roles of mitochondria in cancer targeting therapeutics.
title_full Roles of mitochondria in cancer targeting therapeutics.
title_fullStr Roles of mitochondria in cancer targeting therapeutics.
title_full_unstemmed Roles of mitochondria in cancer targeting therapeutics.
title_sort roles of mitochondria in cancer targeting therapeutics.
publishDate 2011
url http://hdl.handle.net/10356/45273
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