Cell behaviour on micro-textured surfaces
The surface topology of materials can be modified to improve cell-surface interaction. While this has been done extensively by chemical means, mechanical modifications to surface topology are expected to also affect these interactions and therefore are considered worth investigating. Micro-textur...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/45345 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The surface topology of materials can be modified to improve cell-surface interaction. While this has been done extensively by chemical means, mechanical modifications to surface topology are expected to also affect these interactions and therefore are considered worth investigating.
Micro-textured surfaces on polymethyl methacrylate (PMMA) and SS316L stainless steel were fabricated by micro-cutting and micro-electro-discharge machining (EDM) respectively. The PMMA surface was modified by producing grooves of varying widths and depths; the stainless steel surface was modified with overlapping micro-craters to produce varying surface roughness. The proliferation, morphology and alignment of NIH-3T3 fibroblasts on these micro-textured surfaces was analysed.
It was found that cells aligned along the V-grooved samples, and were randomly-orientated in the U-grooved and on the raw samples. Furthermore, the cells were spindle-shaped on the V-grooved and raw samples, but triangular on the U-grooved samples. Cells proliferated well on the micro-textured stainless steel, and reached confluence on textured surfaces; on smooth areas, the cells generally did not adhere. In both cases, cells proliferated better in the presence of gelatine coating. |
|---|