DNA manipulation and measurement using integrated microelectrodes in microchannels

• Main Author: Chia, Menzies Rong Feng.
• Other Authors: Poenar Daniel Puiu
• Format: Final Year Project
• Language: English
• Published: 2011
• Subjects: DRNTU::Engineering::Electrical and electronic engineering::Control and instrumentation::Medical electronics
• Online Access: http://hdl.handle.net/10356/45688
• Institution: Nanyang Technological University

Electrophoresis is a common separation science technique with principles forming the core of many biochemistry methods such as Southern blot technique. Electrophoresis finds application in the field of cancer diagnosis and gene sequencing. In this project, pioneer research is carried out to first establish experiment procedures then, improvise and enhance current electrophoresis methods. This is done by combining microchip electrophoresis together with capacitively-coupled contactless conductivity detection methods. The results is a highly time efficient setup. The developed setup is used to carry out research in the field of cancer diagnosis that yielded fruitful results as published in conference paper by Advances in Microfluidics and Nanofluidics and Asian-Pacific International Symposium on Lab On Chip (AMNAPLOC).