Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01
97 p.
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2011
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| Online Access: | http://hdl.handle.net/10356/47142 |
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sg-ntu-dr.10356-471422023-03-11T17:03:43Z Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 Balasankar Meera Priyadarshini Chou Siaw Meng School of Mechanical and Aerospace Engineering DRNTU::Engineering 97 p. The antimicrobial activity of western as well as eastern drugs against infections caused by P. aeruginosa has become an important area of study in recent years. This study revolves around the use of an effective western drug (Wl), a plant derived chemical or Eastern antibiotic (El) and their combinations against PA01 strain of P. aeruginosa. The synergistic effect of these drugs against biofilm formation was determined using quantification assay technique and their visual evidence was confirmed by Scanning Electron Microscopy. The results of this study indicate that biofilm formation is inhibited in the presence of El even at a sub minimum inhibitory concentration (MIC) value when compared to Wl treated at the same sub MIC value. This study also confirms the presence of moderate synergism between El and Wl in inhibiting biofilm formation. A parallel experiment involved in the development of resistance by planktonic cells of P. aeruginosa against the same antimicrobial agents was carried out. The results showed promising development of resistance at a very high concentration (10 MIC) of Wl within 3 subcultures, at a moderate concentration (5 MIC) of El within 15 subcultures, both of which were treated separately. Resistance to the combination of these drugs (Wl+El) was developed at a concentration of 5 MIC within 13 subcultures. Master of Science (Biomedical Engineering) 2011-12-27T06:28:49Z 2011-12-27T06:28:49Z 2009 2009 Thesis http://hdl.handle.net/10356/47142 Nanyang Technological University application/pdf |
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DRNTU::Engineering Balasankar Meera Priyadarshini Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| description |
97 p. |
| author2 |
Chou Siaw Meng |
| author_facet |
Chou Siaw Meng Balasankar Meera Priyadarshini |
| format |
Theses and Dissertations |
| author |
Balasankar Meera Priyadarshini |
| author_sort |
Balasankar Meera Priyadarshini |
| title |
Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| title_short |
Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| title_full |
Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| title_fullStr |
Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| title_full_unstemmed |
Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01 |
| title_sort |
effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa pa01 |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10356/47142 |
| _version_ |
1761782012506537984 |