Mechanotransduction for mesenchymal stem cells
107 p.
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2011
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sg-ntu-dr.10356-472962023-03-04T16:30:45Z Mechanotransduction for mesenchymal stem cells Wu, Shucheng. Tan Lay Poh School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials 107 p. This project is focused on the study of mechanotransduction of Human mesenchymal stem cells (hMSC). A mechanotransduction device was custom made to stimulate mechanotransduction to stem cells by stretching, bending or shearing cultured materials. Gelatin immobilized Poly (£-caprolactone) (PCL) was used for mechanotransduction stimulation. The response of hMSC was monitored during the mechanotransduction stimulation while culturing. Upregulation of desmin, MAP2, ALP was observed in single axial stretching condition and Upregulation of NKx2.5 in was observed in bi axial stretching condition. The amplitude of stretching strain was studied by single axial stretching at 1.25%, 2.5%, 5%, 10%, 20% strain. GATA4, NKx2.5, Desmin, Nestin, MAP2 was observed at 1.25% single axial strain stretching. For 2.5% of stretching, gene upregulation of GATA4, desine, MAP2, ALP was observed. It suggests that gene upregulation of hMSC by mechanotransduction is strain dependent. Master of Engineering (MSE) 2011-12-27T06:54:48Z 2011-12-27T06:54:48Z 2009 2009 Thesis http://hdl.handle.net/10356/47296 Nanyang Technological University application/pdf |
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Asia |
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Singapore Singapore |
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DRNTU::Engineering::Materials::Biomaterials |
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DRNTU::Engineering::Materials::Biomaterials Wu, Shucheng. Mechanotransduction for mesenchymal stem cells |
| description |
107 p. |
| author2 |
Tan Lay Poh |
| author_facet |
Tan Lay Poh Wu, Shucheng. |
| format |
Theses and Dissertations |
| author |
Wu, Shucheng. |
| author_sort |
Wu, Shucheng. |
| title |
Mechanotransduction for mesenchymal stem cells |
| title_short |
Mechanotransduction for mesenchymal stem cells |
| title_full |
Mechanotransduction for mesenchymal stem cells |
| title_fullStr |
Mechanotransduction for mesenchymal stem cells |
| title_full_unstemmed |
Mechanotransduction for mesenchymal stem cells |
| title_sort |
mechanotransduction for mesenchymal stem cells |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10356/47296 |
| _version_ |
1759853071869935616 |