"Transcriptional regulation and transcription factors as novel drugs targets for human malaria".

• Main Author: Sakinah Mulyana.
• Other Authors: Zbynek Bozdech
• Format: Final Year Project
• Language: English
• Published: 2012
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Drug Resistance; DRNTU::Science::Biological sciences::Biochemistry
• Online Access: http://hdl.handle.net/10356/49258
• Institution: Nanyang Technological University

Plasmodium Falciparum has been shown to develop resistance to all classes anti-malarial drugs. More recent cases of resistance to Artemisinin, an effective drug against malaria, have been reported. This will pose a problem as it will be harder to control the spread of malaria. With drug resistance on the rise, there is a need to find new drug targets for the development of new drugs. One possible target is the transcription factor, PF10_0083. In attempt to obtain the protein in its soluble form, a total of 10 constructs were expressed and tested for its solubility after being subjected to different; (i) temperature, (ii) IPTG concentration, (iii) induction time and (iv) different plasmid. Out of the 10 constructs, constructs 225bp and 420bp, seemed to show indication of soluble protein. Further experiments should be done to express the insoluble constructs. For the soluble constructs, the project can progress to the next phase which is to crystallize the protein to further analyze it.