Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.

Analysis of selected target genes of the ciliogenic transcription factor FoxJ1, LIM Yen Wei Cilia are hair-like protrusions extending from membranes. They are formed from basal bodies and elongate to form axonemes, via a process called ciliogenesis. Disruption in proteins involved in motile c...

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Main Author: Lim, Yen Wei.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2012
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Online Access:http://hdl.handle.net/10356/49372
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-493722023-02-28T18:04:02Z Analysis of selected target genes of the ciliogenic transcription factor FoxJ1. Lim, Yen Wei. School of Biological Sciences A*STAR Institute of Molecular and Cell Biology Sudipto Roy DRNTU::Science Analysis of selected target genes of the ciliogenic transcription factor FoxJ1, LIM Yen Wei Cilia are hair-like protrusions extending from membranes. They are formed from basal bodies and elongate to form axonemes, via a process called ciliogenesis. Disruption in proteins involved in motile cilia, such as dynein, result in ciliopathies such as primary ciliary dyskinesia. We are interested in 7 genes downstream of FoxJ1, which is a master regulator of ciliogenesis. We hypothesise that these genes may localize to the cilia, highlighting the importance of these genes in ciliogenesis. RNA of these genes were made and injected into zebrafish embryos. At least one gene, tmprss13b, was found to localize to the cilia via confocal microscopy. The understanding of cilia-related genes is crucial in elucidating the pathway and mechanisms of ciliogenesis. Understanding how these genes work and function in normal circumstances will also make it easier to develop treatments for the different ciliopathies. Bachelor of Science in Biological Sciences 2012-05-18T01:53:23Z 2012-05-18T01:53:23Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49372 en Nanyang Technological University 46 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Lim, Yen Wei.
Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
description Analysis of selected target genes of the ciliogenic transcription factor FoxJ1, LIM Yen Wei Cilia are hair-like protrusions extending from membranes. They are formed from basal bodies and elongate to form axonemes, via a process called ciliogenesis. Disruption in proteins involved in motile cilia, such as dynein, result in ciliopathies such as primary ciliary dyskinesia. We are interested in 7 genes downstream of FoxJ1, which is a master regulator of ciliogenesis. We hypothesise that these genes may localize to the cilia, highlighting the importance of these genes in ciliogenesis. RNA of these genes were made and injected into zebrafish embryos. At least one gene, tmprss13b, was found to localize to the cilia via confocal microscopy. The understanding of cilia-related genes is crucial in elucidating the pathway and mechanisms of ciliogenesis. Understanding how these genes work and function in normal circumstances will also make it easier to develop treatments for the different ciliopathies.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Lim, Yen Wei.
format Final Year Project
author Lim, Yen Wei.
author_sort Lim, Yen Wei.
title Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
title_short Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
title_full Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
title_fullStr Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
title_full_unstemmed Analysis of selected target genes of the ciliogenic transcription factor FoxJ1.
title_sort analysis of selected target genes of the ciliogenic transcription factor foxj1.
publishDate 2012
url http://hdl.handle.net/10356/49372
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