Evaluation of a new type of rifamycin-bonded chiral stationary phase in high-performance liquid chromatography.

• Main Author: Pang, Shu Hui.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2012
• Subjects: DRNTU::Science
• Online Access: http://hdl.handle.net/10356/49426
• Institution: Nanyang Technological University

Rifamycin-capped (3-(C-methylcalix[4]resorcinarene)-2-hydroxypropoxy)-propylsilyl-appended silica particles (RMCR-HPS), a new type of substituted methylcalix[4]resorcinarene-bonded chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC), have been synthesized by the treatment of bromoacetate-substituted-(3-(C-methylcalix[4]resorcinarene)-2-hydroxypropoxy)-propylsilyl-appended silica particles (BAMCR-HPS) with rifamycin SB in anhydrous acetonitrile. The stationary phase is characterized by means of elemental analysis. This new CSP has a chiral selector consisting of two chiral recognition sites - rifamycin and methylcalix[4]resorcinarene.These two chiral recognition sites contain many different types of functional groups which provide various interactions with chiral compounds so as to enhance chiral recognition of the new CSP.