Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.

The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important...

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Main Author: Loh, Han Ping.
Other Authors: Peter Rainer Preiser
Format: Final Year Project
Language:English
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10356/49488
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-494882023-02-28T18:02:13Z Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. Loh, Han Ping. Peter Rainer Preiser School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Microorganisms The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important factor in determining whether an erythrocyte is suitable for invasion. Recent findings that ATP and ADP can bind to a nucleotide binding region (PfRH1-NBR) within Plasmodium falciparum reticulocyte-binding-like protein homologue 1(PfRH1) suggested that PfRH1 might serve as an ATP sensor during invasion. In this project, the biological relevance of ATP in the invasion process was investigated. My results showed that there was a reduction in extracellular ATP during early invasion thereby suggesting that ATP was used in this process. In addition, to further characterize the role of PfRH1-NBR, I had purified monoclonal antibodies that were raised against PfRH1-NBR and found that these antibodies can inhibit invasion. These findings suggested that ATP, through its interaction with PfRH1 is an important factor during parasite invasion. Bachelor of Science in Biological Sciences 2012-05-21T02:30:42Z 2012-05-21T02:30:42Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49488 en Nanyang Technological University 29 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Microorganisms
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Microorganisms
Loh, Han Ping.
Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
description The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important factor in determining whether an erythrocyte is suitable for invasion. Recent findings that ATP and ADP can bind to a nucleotide binding region (PfRH1-NBR) within Plasmodium falciparum reticulocyte-binding-like protein homologue 1(PfRH1) suggested that PfRH1 might serve as an ATP sensor during invasion. In this project, the biological relevance of ATP in the invasion process was investigated. My results showed that there was a reduction in extracellular ATP during early invasion thereby suggesting that ATP was used in this process. In addition, to further characterize the role of PfRH1-NBR, I had purified monoclonal antibodies that were raised against PfRH1-NBR and found that these antibodies can inhibit invasion. These findings suggested that ATP, through its interaction with PfRH1 is an important factor during parasite invasion.
author2 Peter Rainer Preiser
author_facet Peter Rainer Preiser
Loh, Han Ping.
format Final Year Project
author Loh, Han Ping.
author_sort Loh, Han Ping.
title Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
title_short Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
title_full Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
title_fullStr Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
title_full_unstemmed Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
title_sort insights into the role of the plasmodium falciparum reticulocyte binding protein rh1 in merozoite invasion.
publishDate 2012
url http://hdl.handle.net/10356/49488
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