Surface enhanced Raman-scattering protein sensor
Raman spectroscopy is used to study vibrational modes in molecules. Depending on the constituent atomic masses and the molecular-bond strengths, each molecule possesses a unique set of vibrational frequencies, which allows it to be identified even from a complex background consisting of different ot...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/49789 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Raman spectroscopy is used to study vibrational modes in molecules. Depending on the constituent atomic masses and the molecular-bond strengths, each molecule possesses a unique set of vibrational frequencies, which allows it to be identified even from a complex background consisting of different other molecular species. In Surface Enhanced Raman Spectroscopy (SERS), unique optical property of metallic (example like Gold (Au) or Silver (Ag)) nano-structures are utilized to generate strong light fields localized near nano-protrusions or nano-crevices on the surface. An analyte placed or absorbed within these “hot-spot” areas would experience a strong excitation, resulting in an amplification of the Raman emission. One can thus use SERS hot-spots as an ultrasensitive sensing element for detecting specific molecules in a complex mixture. The project will investigate the use of SERS for protein sensing in bio-fluids with our collaborators in the Singapore Bio-imaging Consortium. |
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