Metabolic changes of host cells in response to HBV replication
As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and q...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/50533 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and quantitatively analyse the metabolites change of host cell in response to the introduction of HBx gene. Four kinds of metabolites are finally picked out: butanedioic acid, glutamine, lactic acid and D-glucose. The first two molecules are significantly down-regulated while the other two seem to be up-regulated, suggesting that glycolysis of the infected cells is enhanced, while TCAC activity is inhibited. |
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