Metabolic changes of host cells in response to HBV replication
As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and q...
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sg-ntu-dr.10356-505332023-03-03T16:01:36Z Metabolic changes of host cells in response to HBV replication Li, Lingyu Chen Wei Ning, William School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and quantitatively analyse the metabolites change of host cell in response to the introduction of HBx gene. Four kinds of metabolites are finally picked out: butanedioic acid, glutamine, lactic acid and D-glucose. The first two molecules are significantly down-regulated while the other two seem to be up-regulated, suggesting that glycolysis of the infected cells is enhanced, while TCAC activity is inhibited. Master of Science 2012-06-11T09:01:14Z 2012-06-11T09:01:14Z 2012 2012 Thesis http://hdl.handle.net/10356/50533 en 53 p. application/pdf |
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Singapore Singapore |
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DRNTU::Engineering::Chemical engineering Li, Lingyu Metabolic changes of host cells in response to HBV replication |
| description |
As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and quantitatively analyse the metabolites change of host cell in response to the introduction of HBx gene. Four kinds of metabolites are finally picked out: butanedioic acid, glutamine, lactic acid and D-glucose. The first two molecules are significantly down-regulated while the other two seem to be up-regulated, suggesting that glycolysis of the infected cells is enhanced, while TCAC activity is inhibited. |
| author2 |
Chen Wei Ning, William |
| author_facet |
Chen Wei Ning, William Li, Lingyu |
| format |
Theses and Dissertations |
| author |
Li, Lingyu |
| author_sort |
Li, Lingyu |
| title |
Metabolic changes of host cells in response to HBV replication |
| title_short |
Metabolic changes of host cells in response to HBV replication |
| title_full |
Metabolic changes of host cells in response to HBV replication |
| title_fullStr |
Metabolic changes of host cells in response to HBV replication |
| title_full_unstemmed |
Metabolic changes of host cells in response to HBV replication |
| title_sort |
metabolic changes of host cells in response to hbv replication |
| publishDate |
2012 |
| url |
http://hdl.handle.net/10356/50533 |
| _version_ |
1759855000201199616 |