Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement.
Parkinson’s disease (PD) is characterized pathologically by the hallmark presence of Lewy bodies, which are intraneuronal inclusions comprised of chiefly α-synuclein. Studies have illustrated an association between the toxicity induced by over-expression of α-synuclein and PD-related locomotive defi...
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sg-ntu-dr.10356-506552023-02-28T18:06:49Z Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. Choo, Wen Rui. School of Biological Sciences National Neuroscience Institute Ng Chee Hoe DRNTU::Science Parkinson’s disease (PD) is characterized pathologically by the hallmark presence of Lewy bodies, which are intraneuronal inclusions comprised of chiefly α-synuclein. Studies have illustrated an association between the toxicity induced by over-expression of α-synuclein and PD-related locomotive deficiency, but specific mechanisms remains unclear. In this study we observed intriguing lost of climbing abilities that is void of apparent DA neuronal loss in wild-type and mutant α-synuclein, A30P and A53T, transgenic PD flies. Using a mitochondrial-targeting sequence tagged Green fluorescence protein reporter (mito-GFP), we observed aberrant mitochondria dynamics in A30P and A53T flies, rendering their neuronal mitochondrial sizes significantly smaller than comparative control. Results thus suggest that, contrary to conventional belief, DA neuron functional loss instead of overt DA neuronal loss actually accounts for the presentation of PD-associated locomotive dysfunction. Furthermore, we have demonstrated that Epigallocatechin 3-gallate (EGCG), a polyphenol found in Green tea, is able to improve locomotive defects and restore DA neuronal loss through modulation of neuronal mitochondrial dynamics. Bachelor of Science in Biomedical Sciences 2012-08-27T06:45:38Z 2012-08-27T06:45:38Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/50655 en Nanyang Technological University 54 p. application/pdf |
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DRNTU::Science Choo, Wen Rui. Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
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Parkinson’s disease (PD) is characterized pathologically by the hallmark presence of Lewy bodies, which are intraneuronal inclusions comprised of chiefly α-synuclein. Studies have illustrated an association between the toxicity induced by over-expression of α-synuclein and PD-related locomotive deficiency, but specific mechanisms remains unclear. In this study we observed intriguing lost of climbing abilities that is void of apparent DA neuronal loss in wild-type and mutant α-synuclein, A30P and A53T, transgenic PD flies. Using a mitochondrial-targeting sequence tagged Green fluorescence protein reporter (mito-GFP), we observed aberrant mitochondria dynamics in A30P and A53T flies, rendering their neuronal mitochondrial sizes significantly smaller than comparative control. Results thus suggest that, contrary to conventional belief, DA neuron functional loss instead of overt DA neuronal loss actually accounts for the presentation of PD-associated locomotive dysfunction. Furthermore, we have demonstrated that Epigallocatechin 3-gallate (EGCG), a polyphenol found in Green tea, is able to improve locomotive defects and restore DA neuronal loss through modulation of neuronal mitochondrial dynamics. |
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School of Biological Sciences |
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School of Biological Sciences Choo, Wen Rui. |
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Final Year Project |
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Choo, Wen Rui. |
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Choo, Wen Rui. |
title |
Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
title_short |
Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
title_full |
Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
title_fullStr |
Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
title_full_unstemmed |
Green tea catechin, EGCG, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
title_sort |
green tea catechin, egcg, modulates mitochondrial dynamics in dopaminergic neurons of α-synuclein transgenic flies resulting in mobility improvement. |
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2012 |
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http://hdl.handle.net/10356/50655 |
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1759857525790867456 |