Functionalised silica coated nanoparticle hybrids for drug delivery and imaging

In recent years, nanoparticles have been extensively explored for biomedical applications due to their unique properties. Among various nanomaterials, silica-based nanoparticles exhibit superior biocompatibility which attracted much research attention. These particles also provide several advantages...

Full description

Saved in:
Bibliographic Details
Main Author: Yang, Yanmei
Other Authors: Xing Bengang
Format: Theses and Dissertations
Language:English
Published: 2013
Subjects:
Online Access:https://hdl.handle.net/10356/51117
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-51117
record_format dspace
spelling sg-ntu-dr.10356-511172023-02-28T23:33:06Z Functionalised silica coated nanoparticle hybrids for drug delivery and imaging Yang, Yanmei Xing Bengang School of Physical and Mathematical Sciences DRNTU::Science::Biological sciences::Biochemistry In recent years, nanoparticles have been extensively explored for biomedical applications due to their unique properties. Among various nanomaterials, silica-based nanoparticles exhibit superior biocompatibility which attracted much research attention. These particles also provide several advantages such as large functional surface, tunable structures and high spectroscopic transparency. In this research dissertation, we focused on developing novel and convenient drug delivery systems for theranostic applications based on silica-coated nanoparticles. Taking the advantage of functional surface structure property of silica nanoparticles, we designed a silica-coated magnetic nanoparticle platform that doxorubicin-peptide conjugated to the surface through “click” chemistry. The chosen peptide could be cleaved by enzyme which only found in the intracellular compartment. This conjugate nanoparticle system demonstrated highly efficient drug release upon enzyme interactions in vitro and in living cells. This system also showed multiple functions that drug release in the selected cells, fluorescence imaging and magnetic resonance imaging. Since silica nanoparticles exhibit good biocompatibility and high spectroscopic transparency, we employed silica-coated upconversion nanoparticles for light-induced therapeutic agents release in the following chapters. By linking the caged D-luciferin to the particles surface, the obtained conjugates can serve as promising platforms for the near-infrared light-responsive bioluminescent molecules release. This approach takes advantage of upconverted UV emission of upconversion nanoparticles from near-infrared light excitation to trigger the uncaging of D-luciferin from the nanoparticle conjugates. Furthermore, the release process can be monitored either by tracking the enhanced fluorescence intensity of D-luciferin or by measuring the bioluminescence signals in vitro and in vivo. The use of silica-coated upconversion nanoparticles can be extended to gene delivery. The particles functionalized with cationic photocaged linker were used as nanocarriers for siRNA delivery to effectively silencing enhanced green fluorescent protein gene expression. siRNA was adsorbed on the platform through electrostatic attractions which was easily internalization by cells. The release process was initiated by the upconverted UV light from upconversion nanoparticles under near-infrared light exposure. As we known, silica-based nanoparticles have two major types, solid silica nanoparticles which we have been used to coat inorganic core nanoparticles in the previous work, the other one is mesoporous silica nanoparticles. DOCTOR OF PHILOSOPHY (SPMS) 2013-01-24T01:43:58Z 2013-01-24T01:43:58Z 2013 2013 Thesis Yang, Y. (2013). Functionalised silica coated nanoparticle hybrids for drug delivery and imaging. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/51117 10.32657/10356/51117 en 174 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Biochemistry
spellingShingle DRNTU::Science::Biological sciences::Biochemistry
Yang, Yanmei
Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
description In recent years, nanoparticles have been extensively explored for biomedical applications due to their unique properties. Among various nanomaterials, silica-based nanoparticles exhibit superior biocompatibility which attracted much research attention. These particles also provide several advantages such as large functional surface, tunable structures and high spectroscopic transparency. In this research dissertation, we focused on developing novel and convenient drug delivery systems for theranostic applications based on silica-coated nanoparticles. Taking the advantage of functional surface structure property of silica nanoparticles, we designed a silica-coated magnetic nanoparticle platform that doxorubicin-peptide conjugated to the surface through “click” chemistry. The chosen peptide could be cleaved by enzyme which only found in the intracellular compartment. This conjugate nanoparticle system demonstrated highly efficient drug release upon enzyme interactions in vitro and in living cells. This system also showed multiple functions that drug release in the selected cells, fluorescence imaging and magnetic resonance imaging. Since silica nanoparticles exhibit good biocompatibility and high spectroscopic transparency, we employed silica-coated upconversion nanoparticles for light-induced therapeutic agents release in the following chapters. By linking the caged D-luciferin to the particles surface, the obtained conjugates can serve as promising platforms for the near-infrared light-responsive bioluminescent molecules release. This approach takes advantage of upconverted UV emission of upconversion nanoparticles from near-infrared light excitation to trigger the uncaging of D-luciferin from the nanoparticle conjugates. Furthermore, the release process can be monitored either by tracking the enhanced fluorescence intensity of D-luciferin or by measuring the bioluminescence signals in vitro and in vivo. The use of silica-coated upconversion nanoparticles can be extended to gene delivery. The particles functionalized with cationic photocaged linker were used as nanocarriers for siRNA delivery to effectively silencing enhanced green fluorescent protein gene expression. siRNA was adsorbed on the platform through electrostatic attractions which was easily internalization by cells. The release process was initiated by the upconverted UV light from upconversion nanoparticles under near-infrared light exposure. As we known, silica-based nanoparticles have two major types, solid silica nanoparticles which we have been used to coat inorganic core nanoparticles in the previous work, the other one is mesoporous silica nanoparticles.
author2 Xing Bengang
author_facet Xing Bengang
Yang, Yanmei
format Theses and Dissertations
author Yang, Yanmei
author_sort Yang, Yanmei
title Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
title_short Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
title_full Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
title_fullStr Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
title_full_unstemmed Functionalised silica coated nanoparticle hybrids for drug delivery and imaging
title_sort functionalised silica coated nanoparticle hybrids for drug delivery and imaging
publishDate 2013
url https://hdl.handle.net/10356/51117
_version_ 1759853286922387456