Investigation of the flow inside micro-PCR device
The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. I...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/53597 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. In this project, the author aims to discuss about the PCR, the details of designing to fabricating of the micro PCR device, follow by testing and analyzing the results. Experiments are carried out to investigate the speed limit for which the DNA samples travel together between the temperature heating zones and the speed limit at which the first DNA sample starts to disperse. Furthermore, the properties of the samples are varied to investigate the effects at room temperature. Last but not least, the discussion on future work focused on the improvements on the design of micro-PCR device to achieve maximum efficacy in PCR. |
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