Investigation of the flow inside micro-PCR device
The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. I...
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sg-ntu-dr.10356-535972023-03-04T19:07:50Z Investigation of the flow inside micro-PCR device Tan, Hui Lin. Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. In this project, the author aims to discuss about the PCR, the details of designing to fabricating of the micro PCR device, follow by testing and analyzing the results. Experiments are carried out to investigate the speed limit for which the DNA samples travel together between the temperature heating zones and the speed limit at which the first DNA sample starts to disperse. Furthermore, the properties of the samples are varied to investigate the effects at room temperature. Last but not least, the discussion on future work focused on the improvements on the design of micro-PCR device to achieve maximum efficacy in PCR. Bachelor of Engineering (Mechanical Engineering) 2013-06-05T08:43:15Z 2013-06-05T08:43:15Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/53597 en Nanyang Technological University 85 p. application/pdf |
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DRNTU::Engineering Tan, Hui Lin. Investigation of the flow inside micro-PCR device |
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The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. In this project, the author aims to discuss about the PCR, the details of designing to fabricating of the micro PCR device, follow by testing and analyzing the results. Experiments are carried out to investigate the speed limit for which the DNA samples travel together between the temperature heating zones and the speed limit at which the first DNA sample starts to disperse. Furthermore, the properties of the samples are varied to investigate the effects at room temperature. Last but not least, the discussion on future work focused on the improvements on the design of micro-PCR device to achieve maximum efficacy in PCR. |
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Gong Haiqing, Thomas |
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Gong Haiqing, Thomas Tan, Hui Lin. |
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Final Year Project |
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Tan, Hui Lin. |
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Tan, Hui Lin. |
title |
Investigation of the flow inside micro-PCR device |
title_short |
Investigation of the flow inside micro-PCR device |
title_full |
Investigation of the flow inside micro-PCR device |
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Investigation of the flow inside micro-PCR device |
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Investigation of the flow inside micro-PCR device |
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investigation of the flow inside micro-pcr device |
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2013 |
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http://hdl.handle.net/10356/53597 |
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1759857453048004608 |