Investigation of the flow inside micro-PCR device

The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. I...

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Main Author: Tan, Hui Lin.
Other Authors: Gong Haiqing, Thomas
Format: Final Year Project
Language:English
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/10356/53597
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-535972023-03-04T19:07:50Z Investigation of the flow inside micro-PCR device Tan, Hui Lin. Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. In this project, the author aims to discuss about the PCR, the details of designing to fabricating of the micro PCR device, follow by testing and analyzing the results. Experiments are carried out to investigate the speed limit for which the DNA samples travel together between the temperature heating zones and the speed limit at which the first DNA sample starts to disperse. Furthermore, the properties of the samples are varied to investigate the effects at room temperature. Last but not least, the discussion on future work focused on the improvements on the design of micro-PCR device to achieve maximum efficacy in PCR. Bachelor of Engineering (Mechanical Engineering) 2013-06-05T08:43:15Z 2013-06-05T08:43:15Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/53597 en Nanyang Technological University 85 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Tan, Hui Lin.
Investigation of the flow inside micro-PCR device
description The Polymerase Chain Reaction (PCR) is a newer technique developed by Kary Mullis in 1983 for amplifying DNA samples. There are many researches about integrating Micro-electro-mechanical (MEMS) technology into the biochip which is essential for speeding up and enhancing the performance of the PCR. In this project, the author aims to discuss about the PCR, the details of designing to fabricating of the micro PCR device, follow by testing and analyzing the results. Experiments are carried out to investigate the speed limit for which the DNA samples travel together between the temperature heating zones and the speed limit at which the first DNA sample starts to disperse. Furthermore, the properties of the samples are varied to investigate the effects at room temperature. Last but not least, the discussion on future work focused on the improvements on the design of micro-PCR device to achieve maximum efficacy in PCR.
author2 Gong Haiqing, Thomas
author_facet Gong Haiqing, Thomas
Tan, Hui Lin.
format Final Year Project
author Tan, Hui Lin.
author_sort Tan, Hui Lin.
title Investigation of the flow inside micro-PCR device
title_short Investigation of the flow inside micro-PCR device
title_full Investigation of the flow inside micro-PCR device
title_fullStr Investigation of the flow inside micro-PCR device
title_full_unstemmed Investigation of the flow inside micro-PCR device
title_sort investigation of the flow inside micro-pcr device
publishDate 2013
url http://hdl.handle.net/10356/53597
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